Food and Processing Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, China.
College of Food Science, Shenyang Agricultural University, Shenyang, China.
Sci Rep. 2022 Feb 8;12(1):2110. doi: 10.1038/s41598-022-05676-z.
This study aimed to characterize the physicochemical properties and stability of L-25 melanin extracted from Sporisorium reilianum (S. reiliana). The results showed that the maximum absorption wavelength of melanin was 215 nm. Reducing agents, heat, light, microwaving, oxidants, and common food additives did not affect the melanin. Additionally, it has a good metal stability except Mn. The IR spectra revealed the presence of O-H, N-H, C=O, and C=C bonds as well as carboxyl, alcohol hydroxyl, and phenolic hydroxyl groups and a pyran ring. L-25 melanin could be defined as DL-hydroxy phenylalanine (DOPA)-melanin. The antioxidant and antiproliferative were also measured. The melanin has a specific stability and high antioxidant activity, including a strong DPPH free radical scavenging ability, and protected damaged HepG2 cells by reducing reactive oxygen species, malondialdehyde, and lactate dehydrogenase content. In conclusion, S. reilianum represents a novel source of melanin, that could be applied to health food or food additives. Our results show that melanin from S. reilianum is a natural pigment with good stability that has a great prospect of development and application, providing a theoretical basis and methods for its further processing and development as a functional food.
本研究旨在表征从米黑毛霉（S. reiliana）中提取的 L-25 黑色素的物理化学性质和稳定性。结果表明，黑色素的最大吸收波长为 215nm。还原剂、热、光、微波、氧化剂和常见的食品添加剂均不影响黑色素。此外，它除了 Mn 之外还具有良好的金属稳定性。IR 光谱显示存在 O-H、N-H、C=O 和 C=C 键以及羧基、醇羟基和酚羟基以及吡喃环。L-25 黑色素可定义为 DL-羟基苯丙氨酸（DOPA）-黑色素。还测量了抗氧化和抗增殖作用。黑色素具有特定的稳定性和高抗氧化活性，包括具有强 DPPH 自由基清除能力，通过降低活性氧、丙二醛和乳酸脱氢酶含量来保护受损的 HepG2 细胞。总之，米黑毛霉代表了一种新型的黑色素来源，可应用于保健食品或食品添加剂。我们的结果表明，米黑毛霉黑色素是一种稳定性良好的天然色素，具有很大的开发和应用前景，为其进一步加工和开发作为功能性食品提供了理论依据和方法。
