National Research and Development Center for Edible Fungi Processing (Wuhan), Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, People's Republic of China.
National Research and Development Center for Edible Fungi Processing (Wuhan), Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, People's Republic of China; Hubei Provincial Engineering Research Center of Under Forest Economy, Hubei Academy of Agricultural Sciences, Wuhan 430064, People's Republic of China.
Int J Med Mushrooms. 2022;24(1):67-82. doi: 10.1615/IntJMedMushrooms.2021041894.
In this study, melanin from wild Auricularia auricula (WAA) was isolated using an ultra-high pressure (UHP)-assisted extraction method, and the physicochemical properties and antioxidant activity of WAA melanin were investigated. Under the optimized extraction conditions of a solid/liquid ratio of 1:30, a UHP of 450 MPa, a 22-min pressure holding time, a 1-mol/L NaOH concentration, and acid precipitation for 8 h, the WAA melanin extraction yield was 7.9 ± 0.16%. Moreover, the results showed that the surface of WAA melanin lacked structural order. Most melanin showed an average diameter of 1000 nm. WAA melanin had strong absorption at a wavelength of 210 nm and displayed typical characteristic absorption peaks. Moreover, WAA melanin contained 48.51% C, 6.88% H, 5.26% N, 0.45% S, and 38.90% O and may be a 3,4-dihydroxyphenylalanine melanin. An analysis of physicochemical properties showed that WAA melanin had good stability toward heat, light, and low concentrations of reducing agents and oxidizing agents. Furthermore, WAA melanin presented certain free radical scavenging activity. This study demonstrates that wild A. auricula melanin may have potential applications in the cosmetic or food industries as a natural antioxidant.
在这项研究中，使用超高压（UHP）辅助提取方法从野生银耳（WAA）中分离出黑色素，并研究了 WAA 黑色素的物理化学性质和抗氧化活性。在优化的提取条件下，固液比为 1:30、UHP 为 450 MPa、保压时间为 22 分钟、1 mol/L NaOH 浓度和酸沉淀 8 小时，WAA 黑色素的提取率为 7.9±0.16%。此外，结果表明 WAA 黑色素表面缺乏结构有序性。大多数黑色素的平均直径为 1000nm。WAA 黑色素在 210nm 波长处具有很强的吸收，并显示出典型的特征吸收峰。此外，WAA 黑色素含有 48.51% C、6.88% H、5.26% N、0.45% S 和 38.90% O，可能是 3,4-二羟基苯丙氨酸黑色素。物理化学性质分析表明，WAA 黑色素对热、光和低浓度的还原剂和氧化剂具有良好的稳定性。此外，WAA 黑色素具有一定的自由基清除活性。这项研究表明，野生银耳黑色素可能作为一种天然抗氧化剂在化妆品或食品工业中有潜在的应用。
