Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions, Institute of Entomology, Guizhou University, Guiyang, 550025, Guizhou, China.
Department of Agronomy, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Sci Rep. 2022 Feb 8;12(1):2141. doi: 10.1038/s41598-022-06193-9.
Parental RNAi (pRNAi) is a response of RNA interference in which treated insect pests progenies showed a gene silencing phenotypes. pRNAi of CmGNA gene has been studied in Cnaphalocrocis medinalis via injection. Our results showed significant reduction in ovulation per female that was 26% and 35.26% in G1 and G2 generations, respectively. Significant reduction of hatched eggs per female were observed 23.53% and 45.26% as compared to control in G1-G2 generations, respectively. We also observed the significant variation in the sex ratio between female (40% and 53%) in G1-G2 generations, and in male (65%) in G1 generation as compared to control. Our results also demonstrated the significant larval mortality (63% and 55%) and pupal mortality (55% and 41%), and significant reduction of mRNA expression level in G1 and G2 generations. Our findings have confirmed that effectiveness of pRNAi induced silencing on the CmGNA target gene in G1-G2 generations of C. medinalis. These results suggested the potential role of pRNAi in insect pest resistance management strategies.
母体 RNAi（pRNAi）是一种 RNA 干扰的反应，经处理的害虫后代表现出基因沉默表型。通过注射已经研究了 Cnaphalocrocis medinalis 中的 CmGNA 基因的 pRNAi。我们的结果显示，每只雌虫的产卵量分别显著减少了 26%和 35.26%，分别在 G1 和 G2 代。与对照组相比，G1-G2 代每只雌虫孵化的卵数分别显著减少了 23.53%和 45.26%。我们还观察到，G1-G2 代雌虫（40%和 53%）和 G1 代雄虫（65%）的性别比例与对照组相比有显著差异。我们的结果还表明，G1 和 G2 代幼虫死亡率（63%和 55%）和蛹死亡率（55%和 41%）显著，mRNA 表达水平也显著降低。我们的研究结果证实了 pRNAi 在 C. medinalis G1-G2 代对 CmGNA 靶基因的诱导沉默的有效性。这些结果表明 pRNAi 在害虫抗管理策略中具有潜在的作用。
