Yu Hai-Zhong, Wen De-Fu, Wang Wan-Lin, Geng Lei, Zhang Yan, Xu Jia-Ping
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Int J Mol Sci. 2015 Sep 10;16(9):21873-96. doi: 10.3390/ijms160921873.
The rice leaf roller (Cnaphalocrocis medinalis) is one of the most destructive agricultural pests. Due to its migratory behavior, it is difficult to control worldwide. To date, little is known about major genes of C. medinalis involved in chitin metabolism and insecticide detoxification. In order to obtain a comprehensive genome dataset of C. medinalis, we conducted de novo transcriptome sequencing which focused on the major feeding stage of fourth-instar larvae, and our work revealed useful information on chitin metabolism and insecticide detoxification and target genes of C. medinalis. We acquired 29,367,797 Illumina reads and assembled these reads into 63,174 unigenes with an average length of 753 bp. Among these unigenes, 31,810 were annotated against the National Center for Biotechnology Information non-redundant (NCBI nr) protein database, resulting in 24,246, 8669 and 18,176 assigned to Swiss-Prot, clusters of orthologous group (COG), and gene ontology (GO), respectively. We were able to map 10,043 unigenes into 285 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG). Specifically, 16 genes, including five chitin deacetylases, two chitin synthases, five chitinases and four other related enzymes, were identified to be putatively involved in chitin biosynthesis and degradation, whereas 360 genes, including cytochrome P450s, glutathione S-transferases, esterases, and acetylcholinesterases, were found to be potentially involved in insecticide detoxification or as insecticide targets. The reliability of the transcriptome data was determined by reverse transcription quantitative PCR (RT-qPCR) for the selected genes. Our data serves as a new and valuable sequence resource for genomic studies on C. medinalis. The findings should improve our understanding of C. medinalis genetics and contribute to management of this important agricultural pest.
稻纵卷叶螟（Cnaphalocrocis medinalis）是最具破坏性的农业害虫之一。由于其迁飞行为，在全球范围内难以防治。迄今为止，关于稻纵卷叶螟参与几丁质代谢和杀虫剂解毒的主要基因知之甚少。为了获得稻纵卷叶螟全面的基因组数据集，我们进行了从头转录组测序，重点关注四龄幼虫的主要取食阶段，我们的研究揭示了有关稻纵卷叶螟几丁质代谢、杀虫剂解毒和靶基因的有用信息。我们获得了29367797条Illumina读数，并将这些读数组装成63174个单基因，平均长度为753 bp。在这些单基因中，31810个与美国国立生物技术信息中心非冗余（NCBI nr）蛋白质数据库进行比对，分别有24246、8669和18176个被分配到Swiss-Prot、直系同源簇（COG）和基因本体（GO）。我们能够使用京都基因与基因组百科全书通路数据库（KEGG）将10043个单基因映射到285条通路中。具体而言，鉴定出16个基因，包括5个几丁质脱乙酰酶、2个几丁质合成酶、5个几丁质酶和4个其他相关酶，推测它们参与几丁质的生物合成和降解，而360个基因，包括细胞色素P450、谷胱甘肽S-转移酶、酯酶和乙酰胆碱酯酶，被发现可能参与杀虫剂解毒或作为杀虫剂靶标。通过对选定基因进行逆转录定量PCR（RT-qPCR）来确定转录组数据的可靠性。我们的数据为稻纵卷叶螟的基因组研究提供了新的有价值的序列资源。这些发现应能增进我们对稻纵卷叶螟遗传学的理解，并有助于对这种重要农业害虫的治理。
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