接受抑制性抗逆转录病毒疗法的个体中多种 HIV-1 持续存在指标之间的关联。
Associations Between Multiple Measures of HIV-1 Persistence in Persons on Suppressive Antiretroviral Therapy.
机构信息
Center for Biostatistics in AIDS Research, Harvard TH Chan School of Public Health, Boston, Massachusetts, USA.
Infectious Diseases Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
出版信息
J Infect Dis. 2022 Jun 15;225(12):2163-2166. doi: 10.1093/infdis/jiac030.
Abstract
Clinical research to achieve antiretroviral therapy-free remission requires quantitative assays of the HIV-1 reservoir. Intact proviral DNA (IPD) measurement has greater throughput than the quantitative viral outgrowth assay (QVOA). In 25 individuals with well-documented long-term viral suppression, IPD levels and infectious units per million CD4+ T cells by QVOA strongly correlated (r = 0.59, P = .002), and IPD correlated with total cell-associated HIV-1 DNA and cell-associated HIV-1 RNA (r = 0.62 and r = 0.59, P ≤ .002). IPD may provide an accessible marker of inducible replication-competent virus, total numbers of infected cells, and cellular expression of HIV-1 RNA.
摘要
临床研究要实现无抗逆转录病毒治疗缓解，需要对 HIV-1 储存库进行定量检测。完整的前病毒 DNA（IPD）测量比定量病毒扩增检测（QVOA）具有更高的通量。在 25 名长期病毒抑制记录良好的个体中，IPD 水平和通过 QVOA 检测到的每百万 CD4+ T 细胞中的感染性单位强烈相关（r=0.59，P=0.002），并且 IPD 与总细胞相关 HIV-1 DNA 和细胞相关 HIV-1 RNA 相关（r=0.62 和 r=0.59，P≤0.002）。IPD 可能提供可及的诱导复制能力病毒、受感染细胞总数以及 HIV-1 RNA 细胞表达的标志物。
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