Center for Biostatistics in AIDS Research, Harvard TH Chan School of Public Health, Boston, Massachusetts, USA.
Infectious Diseases Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
J Infect Dis. 2022 Jun 15;225(12):2163-2166. doi: 10.1093/infdis/jiac030.
Clinical research to achieve antiretroviral therapy-free remission requires quantitative assays of the HIV-1 reservoir. Intact proviral DNA (IPD) measurement has greater throughput than the quantitative viral outgrowth assay (QVOA). In 25 individuals with well-documented long-term viral suppression, IPD levels and infectious units per million CD4+ T cells by QVOA strongly correlated (r = 0.59, P = .002), and IPD correlated with total cell-associated HIV-1 DNA and cell-associated HIV-1 RNA (r = 0.62 and r = 0.59, P ≤ .002). IPD may provide an accessible marker of inducible replication-competent virus, total numbers of infected cells, and cellular expression of HIV-1 RNA.
临床研究要实现无抗逆转录病毒治疗缓解，需要对 HIV-1 储存库进行定量检测。完整的前病毒 DNA（IPD）测量比定量病毒扩增检测（QVOA）具有更高的通量。在 25 名长期病毒抑制记录良好的个体中，IPD 水平和通过 QVOA 检测到的每百万 CD4+ T 细胞中的感染性单位强烈相关（r=0.59，P=0.002），并且 IPD 与总细胞相关 HIV-1 DNA 和细胞相关 HIV-1 RNA 相关（r=0.62 和 r=0.59，P≤0.002）。IPD 可能提供可及的诱导复制能力病毒、受感染细胞总数以及 HIV-1 RNA 细胞表达的标志物。
