Department of Internal Medicine-Oncology, Sichuan Cancer Hospital, Chengdu City, Sichuan Province, China.
Department of Thoracic Surgery, Sichuan Cancer Hospital, Chengdu City, Sichuan Province, China.
Bioengineered. 2022 Feb;13(2):4340-4346. doi: 10.1080/21655979.2022.2031672.
Long noncoding RNA LINRIS (LINC00920) is known to participate in colorectal cancer. This study aimed to explore the role of LINRIS in non-small cell lung cancer (NSCLC). NSCLC and adjacent non-tumor tissues were collected from 62 NSCLC patients. LINRIS expression was detected using real-time quantitative PCR (RT-qPCRs). The 62 NSCLC patients were monitored every month for 5 years to evaluate the role of LINRIS in predicting the prognosis of NSCLC. The effects of LINRIS silencing on microRNA-10a (miR-10a) precursor and mature miR-10a levels were assessed by RT-qPCR. Cell proliferation was measured using Cell Counting Kit-8 (CCK-8) assays. LINRIS expression was upregulated in NSCLC tissues. High LINRIS levels predicted poor survival of NSCLC patients. LINRIS were positively correlated with mature (miR-10a) levels but not miR-10a precursor. In NSCLC cells, LINRIS silencing showed no role in miR-10a precursor accumulation but downregulated mature miR10a level. Moreover, LINRIS silencing inhibited cell proliferation, while miR-10a overexpression increased cell proliferation and inhibited the role of LINRIS silencing. Overall, LINRIS silencing may inhibit NSCLC cell proliferation by suppressing miR-10a maturation. Non-small cell lung cancer (NSCLC); Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR); LncRNA long intergenic noncoding RNA for IGF2BP2 stability (LINRIS).
长链非编码 RNA LINRIS（LINC00920）已知参与结直肠癌。本研究旨在探讨 LINRIS 在非小细胞肺癌（NSCLC）中的作用。收集了 62 例 NSCLC 患者的 NSCLC 组织和相邻非肿瘤组织。采用实时定量 PCR（RT-qPCRs）检测 LINRIS 表达。对 62 例 NSCLC 患者进行每月监测 5 年，以评估 LINRIS 在预测 NSCLC 预后中的作用。通过 RT-qPCR 评估 LINRIS 沉默对 microRNA-10a（miR-10a）前体和成熟 miR-10a 水平的影响。采用细胞计数试剂盒-8（CCK-8）测定细胞增殖。LINRIS 在 NSCLC 组织中表达上调。LINRIS 水平高预示 NSCLC 患者预后不良。LINRIS 与成熟（miR-10a）水平呈正相关，但与 miR-10a 前体无关。在 NSCLC 细胞中，LINRIS 沉默对 miR-10a 前体积累没有作用，但下调成熟 miR10a 水平。此外，LINRIS 沉默抑制细胞增殖，而 miR-10a 过表达增加细胞增殖并抑制 LINRIS 沉默的作用。总体而言，LINRIS 沉默可能通过抑制 miR-10a 成熟来抑制 NSCLC 细胞增殖。非小细胞肺癌（NSCLC）；逆转录定量聚合酶链反应（RT-qPCR）；胰岛素样生长因子 2 mRNA 结合蛋白 2 稳定性的长基因间非编码 RNA（LINRIS）。
