Boni I V, Isaeva D M, Budovskiĭ E I
Bioorg Khim. 1986 Feb;12(2):293-6.
The MS2 RNA fragments bound to ribosomal protein S1 within the complex of MS2 RNA with 30S ribosomal subunit have been isolated using a specially developed procedure and sequenced by the base-specific enzymatic method. The S1-binding site on MS2 RNA was identified as UUUCUUACAUGACAAAUCCUUGUCAUG and mapped within the replicase gene at positions 2030-2056. This finding suggests that ribosome-MS2 RNA interaction involves at least two different regions of the phage RNA--the internal region of the replicase gene (S1-binding site) and ribosome-binding site of the coat protein gene. The possible spatial proximity between these two regions is discussed.
利用一种专门开发的程序，已分离出在MS2 RNA与30S核糖体亚基的复合物中与核糖体蛋白S1结合的MS2 RNA片段，并通过碱基特异性酶法进行了测序。MS2 RNA上的S1结合位点被鉴定为UUUCUUACAUGACAAAUCCUUGUCAUG，并定位在复制酶基因内2030 - 2056位。这一发现表明核糖体与MS2 RNA的相互作用涉及噬菌体RNA的至少两个不同区域——复制酶基因的内部区域（S1结合位点）和外壳蛋白基因的核糖体结合位点。讨论了这两个区域可能的空间接近性。
