Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, 11566 Cairo, Egypt.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, 11566 Cairo, Egypt.
Food Funct. 2022 Feb 21;13(4):2253-2268. doi: 10.1039/d1fo02988f.
species have a longstanding traditional culinary use, mostly being consumed in the Mediterranean diet as a common herb added to food. is commonly consumed as a herbal tea for memory enhancement. Alzheimer's disease (AD) is the most prevalent form of dementia affecting people worldwide Therefore, the current research aimed to investigate potential therapeutic benefits of (SOL) cultivated in Jordan and (SML) cultivated in Egypt with regard to acetylcholinesterase activity, β-amyloid deposition and oxidative stress associated with scopolamine-induced AD. Metabolite profiling of the ethanol extracts of SOL and SML was performed using UPLC-ESI-MS/MS analysis. Methyl carnosate, carnosic acid, carnosol, rosmanol and salvianolic acids were the major secondary metabolites identified in SOL and SML extracts. In our study, scopolamine (1.14 mg kg, i.p.) was administered for 7 consecutive days to induce memory impairment in rats. SML and SOL (150 and 300 mg kg, p.o.) were tested for their effects to reduce the scopolamine-induced deficits. Donepezil (0.5 mg kg, i.p.) was used as a positive control. Scopolamine induced histopathological changes in rats' prefrontal cortex and hippocampus in addition to β-amyloid plaque deposition. Furthermore, scopolamine treatment promoted oxidative stress and acetylcholinesterase activity. On the other hand, treatment with extracts corrected the histological changes induced by scopolamine and significantly reduced β-amyloid deposition. Moreover, both oxidative stress markers and acetylcholinesterase activity were ameliorated by treatment. Using virtual docking to the active sites of the human acetylcholinesterase crystal structure, salvianolic acid K, rosmarinic acid and salvianolic acid C showed the best fitting binding modes to active sites of acetylcholinesterase. Accordingly, the present study demonstrates the beneficial effects of species from Egypt and Jordan against scopolamine-induced AD-like disorder.
这些物种在传统的烹饪中有着悠久的历史，大多作为地中海饮食中的常见草药添加到食物中。迷迭香通常被用作增强记忆力的草药茶。阿尔茨海默病（AD）是最常见的痴呆症，影响着全球的人们。因此，目前的研究旨在调查在约旦种植的迷迭香（SOL）和在埃及种植的鼠尾草（SML）对乙酰胆碱酯酶活性、β-淀粉样蛋白沉积以及与东莨菪碱诱导的 AD 相关的氧化应激的潜在治疗益处。使用 UPLC-ESI-MS/MS 分析对 SOL 和 SML 的乙醇提取物进行代谢产物分析。在 SOL 和 SML 提取物中鉴定出的主要次生代谢产物有甲基牛儿醇、迷迭香酸、鼠尾草酸、罗勒烯和丹酚酸。在我们的研究中，东莨菪碱（1.14mg/kg，腹腔注射）连续 7 天给药以诱导大鼠记忆障碍。测试 SML 和 SOL（150 和 300mg/kg，口服）对降低东莨菪碱诱导的缺陷的作用。多奈哌齐（0.5mg/kg，腹腔注射）用作阳性对照。东莨菪碱诱导大鼠前额叶皮层和海马的组织病理学变化，此外还有β-淀粉样斑块沉积。此外，东莨菪碱处理促进氧化应激和乙酰胆碱酯酶活性。另一方面， 提取物处理纠正了东莨菪碱诱导的组织学变化，并显著减少了β-淀粉样蛋白沉积。此外，两种氧化应激标志物和乙酰胆碱酯酶活性都得到了 提取物的改善。使用虚拟对接到人乙酰胆碱酯酶晶体结构的活性部位，丹酚酸 K、迷迭香酸和丹酚酸 C 显示出与乙酰胆碱酯酶活性部位最佳的结合模式。因此，本研究表明来自埃及和约旦的 物种对东莨菪碱诱导的 AD 样疾病具有有益作用。
