School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan.
Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
J Gen Virol. 2022 Feb;103(2). doi: 10.1099/jgv.0.001718.
A novel picornavirus was isolated from the faeces of a diarrhoeic cow using MA-104 cells at the third blind passage. This virus, named Den1/2021/JPN, was completely sequenced using total RNA from the cell culture supernatant by deep sequencing. The genome of Den1/2021/JPN had a standard picornavirus genome organisation with conserved picornaviral motifs. The 5' untranslated region harboured a type-II internal ribosomal entry site. Den1/2021/JPN was most closely related to a bovine parechovirus (Bo_ParV) named cow/2018/4, which has been recently identified in publicly available databases. Phylogenetic analyses and pairwise sequence comparison revealed that Den1/2021/JPN and Bo_ParV cow/2018/4 clustered with parechoviruses and were most closely related to identified in birds of prey, exhibiting nucleotide sequence similarity of 64.2-64.5 %, 58.6-59.7 % and 66.3-66.4 % in the polyprotein, P1 and 2C+3 CD coding regions, respectively. This study presents the first report on the isolation of Bo_ParV. Den1/2021/JPN and Bo_ParV cow/2018/4, which are candidates for a novel species in the genus .
一种新型小核糖核酸病毒是使用 MA-104 细胞在第三次盲传时从腹泻奶牛的粪便中分离出来的。该病毒被命名为 Den1/2021/JPN，使用来自细胞培养上清液的总 RNA 通过深度测序完成了全基因组测序。Den1/2021/JPN 的基因组具有标准的小核糖核酸病毒基因组结构，具有保守的小核糖核酸病毒基序。5'非翻译区含有 II 型内部核糖体进入位点。Den1/2021/JPN 与一种名为 cow/2018/4 的牛肠道细小病毒（Bo_ParV）最为密切相关，该病毒最近在公开数据库中被鉴定。系统进化分析和成对序列比较表明，Den1/2021/JPN 和 Bo_ParV cow/2018/4 与肠道细小病毒聚为一簇，与已鉴定的猛禽肠道细小病毒最为密切相关，在多蛋白、P1 和 2C+3CD 编码区的核苷酸序列相似性分别为 64.2-64.5%、58.6-59.7%和 66.3-66.4%。本研究首次报道了 Bo_ParV 的分离。Den1/2021/JPN 和 Bo_ParV cow/2018/4 是属内新型候选种。
