Sophion Bioscience A/S, Ballerup, Denmark.
School of Pharmacy, University of Reading, Reading, UK.
Adv Exp Med Biol. 2021;1349:21-32. doi: 10.1007/978-981-16-4254-8_2.
Since its development on the cusp of the new millennium, automated patch clamp (APC) technology has matured over the last two decades. The increased throughput it afforded promised a new paradigm in ion channel recordings: It offered the potential to overcome the time-consuming, low-throughput bottleneck arising from manual patch clamp (MPC) investigations. This chapter highlights the advances in technology, showing how APC platforms have 'democratised' ion channel recordings, lowering the technical bar whilst substantially raising throughput. It will describe the background of the seminal first-generation and updates on advances in second-generation platforms. Furthermore, the chapter summarises the advances APC has made in ion channel studies, including finding new tool compounds and medicines. New functionality and applications on APC platforms give ion channel researchers flexible tools to study ion channels with high quality and high throughput.
自新千年之交开发以来，自动化膜片钳（APC）技术在过去二十年中不断成熟。它提高的通量承诺了离子通道记录的一个新范例：它提供了克服手动膜片钳（MPC）研究中耗时且低通量瓶颈的潜力。本章重点介绍了技术进步，展示了 APC 平台如何“民主化”离子通道记录，降低了技术门槛，同时大大提高了通量。它将描述开创性的第一代的背景以及第二代平台的最新进展。此外，本章总结了 APC 在离子通道研究中的进展，包括发现新的工具化合物和药物。APC 平台的新功能和应用为离子通道研究人员提供了灵活的工具，可高质量和高通量地研究离子通道。
