Laboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia State, 40110-100, Brazil.
Laboratory of Molecular and Cellular Genetics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais State, 31270-901, Brazil.
Braz J Microbiol. 2022 Jun;53(2):1019-1027. doi: 10.1007/s42770-022-00694-5. Epub 2022 Feb 9.
Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis. The main clinical sign of this disease is the development of granulomas, especially in small ruminants; however, the pathways that are involved in the formation and maintenance of these granulomas are unknown. Cytokines and chemokines are responsible for the migration of immune cells to specific sites and tissues; therefore, it is possible that chemokines participate in abscess formation. This study aimed to evaluate the induction of chemokine production by two C. pseudotuberculosis strains in a murine model. A highly pathogenic (VD57) and an attenuated (T1) strain of C. pseudotuberculosis, as well as somatic and secreted antigens derived from these strains, was used to stimulate murine splenocytes. Then, the concentrations of the chemokines CCL-2, CCL-3, CCL-4, and CCL-5 and the cytokines IL-1 and TNF were measured in the culture supernatants. The VD57 strain had a higher ability to stimulate the production of chemokines when compared to T1 strain, especially in the early stages of stimulation, which can have an impact on granuloma formation. The T1 lysate antigen was able to stimulate most of the chemokines studied herein when compared to the other antigenic fractions of both strains. These results indicate that C. pseudotuberculosis is a chemokine production inducer, and the bacterial strains differ in their induction pattern, a situation that can be related to the specific behavior of each strain.
类鼻疽伯克霍尔德菌是干酪性淋巴结炎的病原体。该病的主要临床特征是肉芽肿的形成，尤其是在小型反刍动物中；然而，形成和维持这些肉芽肿的途径尚不清楚。细胞因子和趋化因子负责免疫细胞向特定部位和组织的迁移；因此，趋化因子可能参与脓肿的形成。本研究旨在评估两种类鼻疽伯克霍尔德菌菌株在小鼠模型中诱导趋化因子产生的能力。使用高致病性（VD57）和减毒（T1）株类鼻疽伯克霍尔德菌以及源自这些菌株的体细胞和分泌抗原刺激小鼠脾细胞。然后，在培养上清液中测量趋化因子 CCL-2、CCL-3、CCL-4 和 CCL-5 以及细胞因子 IL-1 和 TNF 的浓度。与 T1 株相比，VD57 株刺激趋化因子产生的能力更高，尤其是在刺激的早期阶段，这可能对肉芽肿的形成产生影响。与两种菌株的其他抗原性部分相比，T1 裂解物抗原能够刺激研究中大多数的趋化因子。这些结果表明，类鼻疽伯克霍尔德菌是趋化因子产生的诱导剂，并且细菌株在诱导模式上存在差异，这种情况可能与每个菌株的特定行为有关。
