The present study challenges the assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of powder, using as an animal model. Surface microdischarge cold atmospheric pressure plasma (SMD-CAPP) treatment was 3.3 W discharge power for 7 min. lifespan and egg laying were used as indicators of antioxidant/antiaging potential of (1 mg/mL), when grown with CP-treated [E_SCP] and untreated [E_S], compared with a control [E_0] (non-supplemented with ). According to our results, under both supplemented media [E_SCP and E_S] and for the first 17 days, nematodes experienced an increase in lifespan but without significant differences ( > 0.05) between control and CP-treated. Regarding the assay of the antimicrobial potential of against serovar Typhimurium (infected worms), no significant differences ( > 0.05) were found between the three exposure scenarios (control [S_0]; supplemented media [S_S]; CP-treated supplemented media [S_SCP]). According to present results, CAPP-treatment do not influence negatively the lifespan of but a reduction in the S antiaging potential was found. No modifications in antimicrobial activity seem to be linked to CAPP-processed a.