Pina-Pérez María Consuelo, Úbeda-Manzanaro María, Beyrer Michael, Martínez Antonio, Rodrigo Dolores
Departamento de Microbiología y Ecología, Universitat de València, Burjassot, Valencia, Spain.
Food Engineering Laboratory, Institute of Life Technologies, University of Applied Sciences and Arts Western-Switzerland (HES-SO) Valais-Wallis, Sion, Switzerland.
Front Microbiol. 2022 Jan 24;12:781871. doi: 10.3389/fmicb.2021.781871. eCollection 2021.
The present study challenges the assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of powder, using as an animal model. Surface microdischarge cold atmospheric pressure plasma (SMD-CAPP) treatment was 3.3 W discharge power for 7 min. lifespan and egg laying were used as indicators of antioxidant/antiaging potential of (1 mg/mL), when grown with CP-treated [E_SCP] and untreated [E_S], compared with a control [E_0] (non-supplemented with ). According to our results, under both supplemented media [E_SCP and E_S] and for the first 17 days, nematodes experienced an increase in lifespan but without significant differences ( > 0.05) between control and CP-treated. Regarding the assay of the antimicrobial potential of against serovar Typhimurium (infected worms), no significant differences ( > 0.05) were found between the three exposure scenarios (control [S_0]; supplemented media [S_S]; CP-treated supplemented media [S_SCP]). According to present results, CAPP-treatment do not influence negatively the lifespan of but a reduction in the S antiaging potential was found. No modifications in antimicrobial activity seem to be linked to CAPP-processed a.
本研究以动物为模型，对冷大气压力等离子体（CAPP）技术对粉末生物活性（抗氧化/抗老化和抗菌潜力）的评估提出了挑战。表面微放电冷大气压力等离子体（SMD-CAPP）处理的放电功率为3.3 W，持续7分钟。当在经冷等离子体处理的[E_SCP]和未处理的[E_S]条件下培养时，与对照[E_0]（未添加）相比，使用寿命和产卵量作为（1 mg/mL）抗氧化/抗老化潜力的指标。根据我们的结果，在两种添加培养基[E_SCP和E_S]下，在前17天，线虫的寿命有所增加，但对照和经冷等离子体处理的之间无显著差异（P>0.05）。关于对鼠伤寒血清型（感染蠕虫）抗菌潜力的测定，在三种暴露情况下（对照[S_0]；添加培养基[S_S]；经冷等离子体处理的添加培养基[S_SCP]）未发现显著差异（P>0.05）。根据目前的结果，冷大气压力等离子体处理不会对的寿命产生负面影响，但发现其抗老化潜力有所降低。抗菌活性似乎没有因冷大气压力等离子体处理的a而发生改变。
