Occurrence of resistance under sublethal/repeated exposure to cauliflower infusion and infection effects on host test organism.

Resistant bacteria to antimicrobials are increasingly emerging in medical, food industry and livestock environments. The present research work assesses the capability of var Typhimurium to become adapted under the exposure to a natural cauliflower antimicrobial by-product infusion in consecutive repeated exposure cycles. was proposed as in vivo host-test organism to compare possible changes in the virulent pattern of the different rounds treated var Typhimurium and untreated bacterial cells. According to the obtained results, var Typhimurium was able to generate resistance against a repeated exposure to cauliflower by-product infusion 5% (w/v), increasing the resistance with the number of exposed repetitions. Meanwhile, at the first exposure, cauliflower by-product infusion was effective in reducing var Typhimurium (≈1 log cycle), and var Typhimurium became resistant to this natural antimicrobial after the second and third treatment-round and was able to grow (≈1 log cycle). In spite of the increased resistance observed for repeatedly treated bacteria, the present study reveals no changes on infection effects between resistant and untreated var Typhimurium, according to phenotypic parameters evaluation (lifespan duration and egg-laying).