从 COVID-19 患者的 PBMCs 和福尔马林固定尸检组织中进行转录组谱分析。
Transcriptome profiling of PBMCs and formalin-fixed autopsy tissues from COVID-19 patients.
机构信息
Department of Anesthesia, Critical Care & Pain Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.
出版信息
STAR Protoc. 2022 Jan 20;3(1):101156. doi: 10.1016/j.xpro.2022.101156. eCollection 2022 Mar 18.
Here we present an optimized protocol for transcriptome profiling of COVID-19 patient samples, including peripheral blood mononuclear cells (PBMCs) and formalin-fixed paraffin-embedded tissue samples obtained from the lung, liver, heart, kidney, and spleen, with the matched controls. We describe RNA extraction and subsequent transcriptome analysis using NanoString technology of the patient samples. The protocol provides information about sample preparation, RNA extraction, and NanoString profiling and analysis. It can be also applied to differentiated Th17 and Treg subsets or formalin-fixed colon tissue samples. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).
在这里，我们提出了一种优化的 COVID-19 患者样本转录组分析方案，包括来自肺、肝、心、肾和脾的外周血单核细胞（PBMCs）和福尔马林固定石蜡包埋组织样本，以及匹配的对照。我们描述了使用 NanoString 技术对患者样本进行 RNA 提取和随后的转录组分析。该方案提供了有关样本制备、RNA 提取和 NanoString 分析的信息。它也可以应用于分化的 Th17 和 Treg 亚群或福尔马林固定的结肠组织样本。有关该方案使用和执行的完整详细信息，请参阅 Wang 等人（2021 年）。
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