Department of Pathology, Immunology, and Laboratory Medicine, University of Florida Diabetes Institute, College of Medicine, Gainesville, FL 32610, USA.
Department of Pathology, Louisiana State University, New Orleans, LA 70112, USA.
STAR Protoc. 2021 Sep 17;2(3):100696. doi: 10.1016/j.xpro.2021.100696. Epub 2021 Jul 21.
Angiotensin-converting enzyme 2 (ACE2) is a key cellular entry factor for severe acute respiratory syndrome coronavirus 2. Hence, identifying cell types that express ACE2 is important for understanding the pathophysiology of coronavirus disease 2019. We performed extensive testing of multiple primary antibodies across various human tissue types. Here, we describe an optimized protocol for immunostaining of ACE2 in formalin-fixed paraffin-embedded human pancreas, small intestine, and kidney tissue sections obtained from organ donors and autopsies. For complete details on the use and execution of this protocol, please refer to Kusmartseva et al. (2020).
血管紧张素转化酶 2（ACE2）是严重急性呼吸综合征冠状病毒 2 的关键细胞进入因子。因此，鉴定表达 ACE2 的细胞类型对于了解 2019 年冠状病毒病的病理生理学很重要。我们对多种人类组织类型的多种主要抗体进行了广泛测试。在这里，我们描述了一种优化的免疫组化方案，用于对从器官捐献者和尸检获得的福尔马林固定石蜡包埋的人胰腺、小肠和肾脏组织切片中的 ACE2 进行染色。有关此方案使用和执行的完整详细信息，请参见 Kusmartseva 等人（2020 年）。
