DU Qiuli, Wang Chao, Liu Guanwen, Zhang Dandan, Zhang Shujun, Qiu Jinlong
Department of Life Science and Engineering, Jining University, Qufu 273155, Shandong, China.
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Jan 25;38(1):26-33. doi: 10.13345/j.cjb.210291.
The CRISPR/Cas9 based prime editing (PE) technique enables all 12 types of base substitutions and precise small DNA deletions or insertions without generating DNA double-strand breaks. Prime editing has been successfully applied in plants and plays important roles in plant precision breeding. Although plant prime editing (PPE) can substantially expand the scope and capabilities of precise genome editing in plants, its editing efficiency still needs to be further improved. Here, we review the development of PPE technique, and introduce structural composition, advantages and limitations of PPE. Strategies to improve the PPE editing efficiency, including the -directed PBS length design, the RT template length, the dual-pegRNA strategy, the PlantPegDesigner website, and the strategies for optimizing the target proteins of PPE, were highlighted. Finally, the prospects of future development and application of PPE were discussed.
基于CRISPR/Cas9的引导编辑（PE）技术能够实现所有12种类型的碱基替换以及精确的小片段DNA缺失或插入，而不会产生DNA双链断裂。引导编辑已在植物中成功应用，并在植物精准育种中发挥重要作用。尽管植物引导编辑（PPE）能够大幅扩展植物精确基因组编辑的范围和能力，但其编辑效率仍需进一步提高。在此，我们综述了PPE技术的发展，并介绍了PPE的结构组成、优点和局限性。重点介绍了提高PPE编辑效率的策略，包括定向PBS长度设计、RT模板长度、双pegRNA策略、PlantPegDesigner网站以及优化PPE靶蛋白的策略。最后，讨论了PPE未来发展和应用的前景。
