Sun Pingyong, Zhang Wuhan, Shu Fu, He Qiang, Zhang Li, Yang Zhuhong, Peng Zhirong, Xie Yun, Deng Huafeng
State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, Hunan, China.
Nuclear Agriculture and Space Breeding Research Institute, Changsha 410125, Hunan, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Jan 25;38(1):252-263. doi: 10.13345/j.cjb.210401.
Cultivating salt-alkali tolerant rice varieties is one of the important ways to meet the increasing food demand of growing global population. In this study, twenty-one rice germplasms with different salt-alkali tolerance were treated with six salt-alkali concentrations at germination and seedling stages. The germination potential, germination rate, shoot length, root length, root number, fresh weight of shoot and seedlings were measured. The average value of salt damage rate was used to evaluate the salt-alkali tolerance. As the salt-alkali concentration increases, the inhibition on seed germination and growth became more obvious. Upon treatment with 1% NaCl plus 0.25% NaHCO, the salt damage rate of germination rate has the largest variation, ranging from 0% to 89.80%. The salt damage rate of each trait shows a similar trend at all concentrations. Four germplasm resources with strong salt-alkali tolerance (Dajiugu, Nippobare, Mowanggu and 02428) and 7 sensitive germplasms were screened. The salt-tolerant gene sequence of 4 salt-alkali tolerant varieties and 3 sensitive germplasms were analyzed. and were identical among the 7 germplasms, but and showed clear variations between the salt-alkali tolerant and sensitive germplasms. Besides the salt-alkali tolerant germplasm resources, this study can also serve as a reference for mining of genes involved in salt-alkali tolerance and breeding of salt-alkali tolerant rice varieties.
培育耐盐碱水稻品种是满足全球人口增长对粮食需求不断增加的重要途径之一。在本研究中，对21份具有不同耐盐碱能力的水稻种质在发芽期和苗期用6种盐碱浓度进行处理。测定了发芽势、发芽率、苗长、根长、根数、地上部鲜重和幼苗鲜重。用盐害率平均值评价耐盐碱能力。随着盐碱浓度的增加，对种子萌发和生长的抑制作用更加明显。用1%NaCl加0.25%NaHCO₃处理时，发芽率的盐害率变化最大，范围为0%至89.80%。各性状的盐害率在所有浓度下均呈现相似趋势。筛选出4份耐盐碱能力强的种质资源（大酒谷、日本晴、莫王谷和02428）和7份敏感种质。对4个耐盐碱品种和3个敏感种质的耐盐基因序列进行了分析。7份种质中的[具体基因名称1]和[具体基因名称2]相同，但[具体基因名称3]和[具体基因名称4]在耐盐碱和敏感种质之间表现出明显差异。除耐盐碱种质资源外，本研究还可为耐盐碱基因挖掘和耐盐碱水稻品种选育提供参考。
