Liu Chen, Lin Yicheng, Guo Bin, Li Ningyu, Li Hua, Fu Qinglin
Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Jan 25;38(1):287-302. doi: 10.13345/j.cjb.210177.
As a non-essential metal, cadmium (Cd) pollution poses severe threats to plant growth, environment, and human health. Phytoextraction using nursery stocks prior to their transplantation is a potential useful approach for bioremediation of Cd contaminated soil. A greenhouse pot experiment was performed to investigate the growth, Cd accumulation, profiles of transcriptome as well as root-associated microbiomes of in Cd-added soil, upon inoculation of two types of arbuscular mycorrhizal fungi (AMF) and . Compared with the control, inoculation of increased Cd concentrations in root, stem and leaf by 57.2%, 44.1% and 71.1%, respectively, contributing to a total Cd content of 182 μg/plant. KEGG pathway analysis revealed that hundreds of genes involved in 'Mitogen-activated protein kinase (MAPK) signaling pathway', 'plant hormone signal transduction', 'biosynthesis of secondary metabolites' and 'glycolysis/gluconeogenesis' were enriched upon inoculation of . The relative abundance of Acidobacteria was increased upon inoculation of , while Chloroflexi and Patescibacteria were increased upon inoculation of , and the abundance of Glomerales increased from 23.0% to above 70%. Correlation analysis indicated that ethylene-responsive transcription factor, alpha-aminoadipic semialdehyde synthase, isoamylase and agmatine deiminase related genes were negatively associated with the relative abundance of Glomerales operational taxonomic units (OTUs) upon inoculation of . In addition, plant cysteine oxidase, heat shock protein, cinnamoyl-CoA reductase and abscisic acid receptor related genes were positively associated with the relative abundance of Patescibacteria OTUs upon inoculation of . These finding suggested that AMF can enhance Cd uptake by modulating plant gene expression and altering the structure of the soil microbial community. This study provides a theoretical basis for better understanding the relationship between root-associated microbiomes and root transcriptomes of , from which a cost-effective and environment-friendly strategy for phytoextraction of Cd in Cd-polluted soil might be developed.
作为一种非必需金属，镉（Cd）污染对植物生长、环境和人类健康构成严重威胁。在苗木移植前利用其进行植物提取是镉污染土壤生物修复的一种潜在有效方法。进行了一项温室盆栽试验，以研究在添加镉的土壤中接种两种丛枝菌根真菌（AMF） 和 后， 的生长、镉积累、转录组图谱以及根际微生物群落。与对照相比，接种 后根、茎和叶中的镉浓度分别增加了57.2%、44.1%和71.1%，单株总镉含量达到182μg。KEGG通路分析表明，接种 后，数百个参与“丝裂原活化蛋白激酶（MAPK）信号通路”、“植物激素信号转导”、“次生代谢物生物合成”和“糖酵解/糖异生”的基因得到富集。接种 后酸杆菌的相对丰度增加，接种 后绿弯菌门和Patescibacteria增加，球囊菌纲的丰度从23.0%增加到70%以上。相关性分析表明，接种 后，乙烯响应转录因子、α-氨基己二酸半醛合酶、异淀粉酶和胍丁胺脱亚氨酶相关基因与球囊菌纲操作分类单元（OTU）的相对丰度呈负相关。此外，接种 后，植物半胱氨酸氧化酶、热休克蛋白、肉桂酰辅酶A还原酶和脱落酸受体相关基因与Patescibacteria OTU的相对丰度呈正相关。这些发现表明，AMF可以通过调节植物基因表达和改变土壤微生物群落结构来增强 对镉的吸收。本研究为更好地理解 的根际微生物群落与根转录组之间的关系提供了理论基础，据此可能开发出一种经济高效且环境友好的镉污染土壤植物提取策略。
