Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology, Beijing 100124, P. R. China.
Cuiying Biomedical Research Center, Lanzhou University Second Hospital, Lanzhou 730030, P. R. China.
Chem Commun (Camb). 2022 Feb 24;58(17):2926-2929. doi: 10.1039/d1cc07067c.
A narrow-bore capillary (inner diameter = 2 μm) zone electrophoresis method was developed to study the interaction between metformin hydrochloride and bovine serum albumin. Free metformin hydrochloride, free bovine serum albumin, and their complex were completely separated in a volume of tens of picoliters. The association constant ( = 1.04 × 10 L mol) and the number of binding sites ( = 0.9789) were then obtained.
建立了一种窄口径毛细管（内径=2μm）区带电泳方法，用于研究盐酸二甲双胍与牛血清白蛋白之间的相互作用。在几十皮升的体积中，完全分离了游离的盐酸二甲双胍、游离的牛血清白蛋白及其复合物。然后得到了结合常数（=1.04×10 L mol）和结合位点数（=0.9789）。
