肠致病性大肠杆菌对人喉表皮样癌细胞（HEp-2）黏附决定簇的定位
Localization of a determinant for HEp-2 adherence by enteropathogenic Escherichia coli.
作者信息
Baldini M M, Nataro J P, Kaper J B
出版信息
Infect Immun. 1986 Apr;52(1):334-6. doi: 10.1128/iai.52.1.334-336.1986.
Abstract
pMAR2, a 60-megadalton plasmid encoding localized HEp-2 adherence in enteropathogenic Escherichia coli, was mapped with BamHI, HindIII, and SalI. Deletion and insertion mutants were constructed and used to define a potential DNA probe. Preliminary results indicate that this probe is sensitive and specific for the genes encoding the enteropathogenic E. coli adherence factor.
摘要
pMAR2是一种60兆道尔顿的质粒，编码肠道致病性大肠杆菌中的局部HEp - 2黏附，用BamHI、HindIII和SalI进行了图谱分析。构建了缺失和插入突变体并用于确定一种潜在的DNA探针。初步结果表明，该探针对于编码肠道致病性大肠杆菌黏附因子的基因具有敏感性和特异性。
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