• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

日本草药半夏泻心汤通过激活细胞外信号调节激酶介导CXCL12的表达来诱导口腔角质形成细胞迁移。

The Japanese Herbal Medicine Hangeshashinto Induces Oral Keratinocyte Migration by Mediating the Expression of CXCL12 Through the Activation of Extracellular Signal-Regulated Kinase.

作者信息

Miyano Kanako, Hasegawa Seiya, Asai Noriho, Uzu Miaki, Yatsuoka Wakako, Ueno Takao, Nonaka Miki, Fujii Hideaki, Uezono Yasuhito

机构信息

Division of Cancer Pathophysiology, National Cancer Research Institute, Tokyo, Japan.

Department of Pain Control Research, The Jikei University School of Medicine, Tokyo, Japan.

出版信息

Front Pharmacol. 2022 Jan 18;12:695039. doi: 10.3389/fphar.2021.695039. eCollection 2021.

DOI:10.3389/fphar.2021.695039
PMID:35145397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8822321/
Abstract

Several clinical studies have reported that Japanese herbal medicine Hangeshashinto (HST) has beneficial effects on chemotherapy-induced oral ulcerative mucositis (OUM). Our previous research demonstrated that HST improves chemotherapy-induced OUM through human oral keratinocyte (HOK) migration, which was suppressed by mitogen-activated protein kinase (MAPK) and C-X-C chemokine receptor 4 (CXCR4) inhibitors. However, the association between these molecules and HOK migration was unclear. Here, we examined the effects of HST on the expression of CXCR4/CXCR7 and C-X-C motif chemokine ligands 11 and 12 (CXCL11/CXCL12) in HOKs. Our results indicated that HST upregulated CXCL12, but not CXCR4, CXCR7, nor CXCL11 in HOKs. HST-induced expression of CXCL12 was significantly suppressed by an inhibitor of extracellular signal-regulated kinase (ERK), but not of p38 and c-Jun N-terminal kinase (JNK). In addition, HST induced phosphorylation of ERK in HOKs. These findings suggest that HST enhances HOK migration by upregulating CXCL12 via ERK.

摘要

多项临床研究报告称，日本草药半夏泻心汤（HST）对化疗引起的口腔溃疡性黏膜炎（OUM）具有有益作用。我们之前的研究表明，HST通过人口腔角质形成细胞（HOK）迁移改善化疗引起的OUM，而丝裂原活化蛋白激酶（MAPK）和C-X-C趋化因子受体4（CXCR4）抑制剂会抑制这种迁移。然而，这些分子与HOK迁移之间的关联尚不清楚。在此，我们研究了HST对HOK中CXCR4/CXCR7以及C-X-C基序趋化因子配体11和12（CXCL11/CXCL12）表达的影响。我们的结果表明，HST上调了HOK中的CXCL12，但未上调CXCR4、CXCR7或CXCL11。细胞外信号调节激酶（ERK）抑制剂可显著抑制HST诱导的CXCL12表达，而p38和c-Jun氨基末端激酶（JNK）抑制剂则无此作用。此外，HST诱导了HOK中ERK的磷酸化。这些发现表明，HST通过ERK上调CXCL12来增强HOK迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/038582a6da8b/fphar-12-695039-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/befe210d85b3/fphar-12-695039-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/8d67c259b17a/fphar-12-695039-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/8af57995ce87/fphar-12-695039-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/895b894ef169/fphar-12-695039-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/f56f407c2653/fphar-12-695039-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/038582a6da8b/fphar-12-695039-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/befe210d85b3/fphar-12-695039-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/8d67c259b17a/fphar-12-695039-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/8af57995ce87/fphar-12-695039-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/895b894ef169/fphar-12-695039-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/f56f407c2653/fphar-12-695039-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c48b/8822321/038582a6da8b/fphar-12-695039-g006.jpg

相似文献

1
The Japanese Herbal Medicine Hangeshashinto Induces Oral Keratinocyte Migration by Mediating the Expression of CXCL12 Through the Activation of Extracellular Signal-Regulated Kinase.日本草药半夏泻心汤通过激活细胞外信号调节激酶介导CXCL12的表达来诱导口腔角质形成细胞迁移。
Front Pharmacol. 2022 Jan 18;12:695039. doi: 10.3389/fphar.2021.695039. eCollection 2021.
2
The Japanese herbal medicine Hangeshashinto enhances oral keratinocyte migration to facilitate healing of chemotherapy-induced oral ulcerative mucositis.日本汉方药黄耆建中汤促进口腔角质细胞迁移，促进化疗诱导的口腔黏膜炎愈合。
Sci Rep. 2020 Jan 17;10(1):625. doi: 10.1038/s41598-019-57192-2.
3
[6]-gingerol and [6]-shogaol, active ingredients of the traditional Japanese medicine hangeshashinto, relief oral ulcerative mucositis-induced pain via action on Na channels.[6]-姜辣素和[6]-姜烯酚是传统日本药物半夏泻心汤的活性成分，通过作用于钠通道缓解口腔溃疡性黏膜炎引起的疼痛。
Pharmacol Res. 2017 Mar;117:288-302. doi: 10.1016/j.phrs.2016.12.026. Epub 2016 Dec 30.
4
Multitargeted effects of hangeshashinto for treatment of chemotherapy-induced oral mucositis on inducible prostaglandin E2 production in human oral keratinocytes.半夏泻心汤治疗化疗引起的口腔黏膜炎对人口腔角质形成细胞中诱导型前列腺素E2产生的多靶点作用。
Integr Cancer Ther. 2014 Sep;13(5):435-45. doi: 10.1177/1534735413520035. Epub 2014 Feb 4.
5
CXCL12 induces connective tissue growth factor expression in human lung fibroblasts through the Rac1/ERK, JNK, and AP-1 pathways.CXCL12通过Rac1/ERK、JNK和AP-1信号通路诱导人肺成纤维细胞中结缔组织生长因子的表达。
PLoS One. 2014 Aug 14;9(8):e104746. doi: 10.1371/journal.pone.0104746. eCollection 2014.
6
Chemokine CXCL12 activates dual CXCR4 and CXCR7-mediated signaling pathways in pancreatic cancer cells.趋化因子 CXCL12 激活胰腺癌细胞中的双重 CXCR4 和 CXCR7 介导的信号通路。
J Transl Med. 2012 Apr 2;10:68. doi: 10.1186/1479-5876-10-68.
7
Comparison between hangeshashinto and dexamethasone for IL-1α and β-defensin 1 production by human oral keratinocytes.汉黄芩素与人口腔角质形成细胞产生的白细胞介素-1α和β-防御素 1 的比较。
J Oral Biosci. 2024 Mar;66(1):188-195. doi: 10.1016/j.job.2024.01.007. Epub 2024 Jan 24.
8
The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects.趋化因子受体 CXCR7 在人神经胶质瘤细胞中高表达，并介导抗细胞凋亡作用。
Cancer Res. 2010 Apr 15;70(8):3299-308. doi: 10.1158/0008-5472.CAN-09-3642. Epub 2010 Apr 13.
9
CXCL12/CXCR4 chemokine signaling in spinal glia induces pain hypersensitivity through MAPKs-mediated neuroinflammation in bone cancer rats.脊髓胶质细胞中的CXCL12/CXCR4趋化因子信号通过丝裂原活化蛋白激酶介导的神经炎症在骨癌大鼠中诱发痛觉过敏。
J Neurochem. 2015 Feb;132(4):452-63. doi: 10.1111/jnc.12985. Epub 2015 Jan 26.
10
Pain mechanism of oral ulcerative mucositis and the therapeutic traditional herbal medicine hangeshashinto.口腔溃疡性黏膜炎的疼痛机制及传统草药半夏泻心汤的治疗作用
J Oral Biosci. 2019 Mar;61(1):12-15. doi: 10.1016/j.job.2019.01.004. Epub 2019 Jan 30.

引用本文的文献

1
Reunderstanding the classical prescription Banxia Xiexin Decoction: new perspectives from a comprehensive review of clinical research and pharmacological studies.重新认识经典方剂半夏泻心汤：基于临床研究与药理研究全面综述的新视角
Chin Med. 2025 Mar 18;20(1):39. doi: 10.1186/s13020-025-01087-0.
2
Consensus statement on chronic pain treatment in cancer survivors.癌症幸存者慢性疼痛治疗共识声明。
J Anesth. 2025 Apr;39(2):161-181. doi: 10.1007/s00540-024-03427-0. Epub 2024 Dec 4.
3
CXCR7 activation evokes the anti-PD-L1 antibody against glioblastoma by remodeling CXCL12-mediated immunity.

本文引用的文献

1
Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration.从人皮肤中分离表皮角质形成细胞：评估表皮角质形成细胞迁移的划痕试验。
Methods Mol Biol. 2020;2154:1-12. doi: 10.1007/978-1-0716-0648-3_1.
2
The Japanese herbal medicine Hangeshashinto enhances oral keratinocyte migration to facilitate healing of chemotherapy-induced oral ulcerative mucositis.日本汉方药黄耆建中汤促进口腔角质细胞迁移，促进化疗诱导的口腔黏膜炎愈合。
Sci Rep. 2020 Jan 17;10(1):625. doi: 10.1038/s41598-019-57192-2.
3
Baicalein induces the apoptosis of HCT116 human colon cancer cells via the upregulation of DEPP/Gadd45a and activation of MAPKs.
CXCR7 激活通过重塑 CXCL12 介导的免疫来引发针对神经胶质瘤的抗 PD-L1 抗体。
Cell Death Dis. 2024 Jun 19;15(6):434. doi: 10.1038/s41419-024-06784-6.
4
Potential of the Novel Slot Blot Method with a PVDF Membrane for Protein Identification and Quantification in Kampo Medicines.用于汉方药中蛋白质鉴定和定量的新型聚偏二氟乙烯膜狭缝印迹法的潜力。
Membranes (Basel). 2023 Dec 1;13(12):896. doi: 10.3390/membranes13120896.
5
Novel In Vitro Assay of the Effects of Kampo Medicines against Intra/Extracellular Advanced Glycation End-Products in Oral, Esophageal, and Gastric Epithelial Cells.汉方药物对口腔、食管和胃上皮细胞内/外晚期糖基化终产物作用的新型体外测定法
Metabolites. 2023 Jul 24;13(7):878. doi: 10.3390/metabo13070878.
黄芩素通过上调 DEPP/Gadd45a 和激活 MAPKs 诱导 HCT116 人结肠癌细胞凋亡。
Int J Oncol. 2018 Aug;53(2):750-760. doi: 10.3892/ijo.2018.4402. Epub 2018 May 11.
4
[6]-gingerol and [6]-shogaol, active ingredients of the traditional Japanese medicine hangeshashinto, relief oral ulcerative mucositis-induced pain via action on Na channels.[6]-姜辣素和[6]-姜烯酚是传统日本药物半夏泻心汤的活性成分，通过作用于钠通道缓解口腔溃疡性黏膜炎引起的疼痛。
Pharmacol Res. 2017 Mar;117:288-302. doi: 10.1016/j.phrs.2016.12.026. Epub 2016 Dec 30.
5
Treatment for Cancer Patients with Oral Mucositis: Assessment Based on the Mucositis Study Group of the Multinational Association of Supportive Care in Cancer in International Society of Oral Oncology (MASCC/ISOO) in 2013 and Proposal of Possible Novel Treatment with a Japanese Herbal Medicine.癌症患者口腔黏膜炎的治疗：基于2013年国际口腔肿瘤学会多国支持性癌症护理协会口腔黏膜炎研究组（MASCC/ISOO）的评估以及日本草药可能的新治疗方案建议。
Curr Pharm Des. 2016;22(15):2270-8. doi: 10.2174/1381612822666160219120842.
6
The traditional Japanese medicine hangeshashinto alleviates oral ulcer-induced pain in a rat model.传统日本药物半夏泻心汤可减轻大鼠模型中口腔溃疡引起的疼痛。
Arch Oral Biol. 2016 Jun;66:30-7. doi: 10.1016/j.archoralbio.2016.02.002. Epub 2016 Feb 3.
7
Sulfated polysaccharide isolated from the sea cucumber Stichopus japonicas promotes the SDF-1α/CXCR4 axis-induced NSC migration via the PI3K/Akt/FOXO3a, ERK/MAPK, and NF-κB signaling pathways.从日本刺参中分离出的硫酸化多糖通过PI3K/Akt/FOXO3a、ERK/MAPK和NF-κB信号通路促进SDF-1α/CXCR4轴诱导的神经干细胞迁移。
Neurosci Lett. 2016 Mar 11;616:57-64. doi: 10.1016/j.neulet.2016.01.041. Epub 2016 Jan 28.
8
Effects of Hangeshashinto on Growth of Oral Microorganisms.半夏泻心汤对口腔微生物生长的影响。
Evid Based Complement Alternat Med. 2015;2015:512947. doi: 10.1155/2015/512947. Epub 2015 Jun 15.
9
Double-blind, placebo-controlled, randomized phase II study of TJ-14 (Hangeshashinto) for infusional fluorinated-pyrimidine-based colorectal cancer chemotherapy-induced oral mucositis.TJ-14（汉方柴芍六君子汤）用于基于氟尿嘧啶输注的结直肠癌化疗所致口腔黏膜炎的双盲、安慰剂对照、随机II期研究。
Cancer Chemother Pharmacol. 2015 Jul;76(1):97-103. doi: 10.1007/s00280-015-2767-y. Epub 2015 May 17.
10
Analysis of the antioxidative function of the radioprotective Japanese traditional (Kampo) medicine, hangeshashinto, in an aqueous phase.对具有辐射防护作用的日本传统（汉方）药物半夏泻心汤水相抗氧化功能的分析。
J Radiat Res. 2015 Jul;56(4):669-77. doi: 10.1093/jrr/rrv023. Epub 2015 Apr 16.