Soltani Samira, Zirah Séverine, Rebuffat Sylvie, Couture Frédéric, Boutin Yvan, Biron Eric, Subirade Muriel, Fliss Ismail
Food Science Department, Food and Agriculture Faculty, Laval University, Quebec, QC, Canada.
Muséum National d'Histoire Naturelle, Centre National de la Recherche Scientifique, Laboratory Molecules of Communication and Adaptation of Microorganisms, UMR 7245 CNRS-MNHN, Paris, France.
Front Microbiol. 2022 Jan 25;12:780355. doi: 10.3389/fmicb.2021.780355. eCollection 2021.
Bacteriocins are receiving increased attention as potent candidates in food preservation and medicine. Although the inhibitory activity of bacteriocins has been studied widely, little is known about their gastrointestinal stability and toxicity toward normal human cell lines. The aim of this study was to evaluate the gastrointestinal stability and activity of microcin J25, pediocin PA-1, bactofencin A and nisin using models. In addition cytotoxicity and hemolytic activity of these bacteriocins were investigated on human epithelial colorectal adenocarcinoma cells (Caco-2) and rat erythrocytes, respectively. Pediocin PA-1, bactofencin A, and nisin were observed to lose their stability while passing through the gastrointestinal tract, while microcin J25 is only partially degraded. Besides, selected bacteriocins were not toxic to Caco-2 cells, and integrity of cell membrane was observed to remain unaffected in presence of these bacteriocins at concentrations up to 400 μg/mL. In hemolysis study, pediocin PA-1, bactofencin A, and nisin were observed to lyse rat erythrocytes at concentrations higher than 50 μg/mL, while microcin J25 showed no effect on these cells. According to data indicating gastrointestinal degradation and the absence of toxicity of pediocin PA-1, bactofencin A, and microcin J25 they could potentially be used in food or clinical applications.
细菌素作为食品保鲜和医药领域的有力候选物正受到越来越多的关注。尽管对细菌素的抑制活性已进行了广泛研究，但关于它们在胃肠道中的稳定性以及对正常人类细胞系的毒性却知之甚少。本研究的目的是使用模型评估微菌素J25、片球菌素PA-1、杆菌防御素A和乳链菌肽在胃肠道中的稳定性和活性。此外，还分别研究了这些细菌素对人上皮结肠腺癌细胞（Caco-2）和大鼠红细胞的细胞毒性及溶血活性。观察到片球菌素PA-1、杆菌防御素A和乳链菌肽在通过胃肠道时失去稳定性，而微菌素J25仅部分降解。此外，所选细菌素对Caco-2细胞无毒，并且在浓度高达400μg/mL的这些细菌素存在下，观察到细胞膜完整性未受影响。在溶血研究中，观察到片球菌素PA-1、杆菌防御素A和乳链菌肽在浓度高于50μg/mL时会使大鼠红细胞裂解，而微菌素J25对这些细胞无影响。根据表明片球菌素PA-1、杆菌防御素A和微菌素J25在胃肠道中降解且无毒性的数据，它们有可能用于食品或临床应用。
