Williams Trevor, López-Ferber Miguel, Caballero Primitivo
Instituto de Ecología AC, Xalapa, Mexico.
Hydrosciences Montpellier, Univ Montpellier, IMT Mines Alès, IRD, CNRS, Alès, France.
Front Microbiol. 2022 Jan 25;12:810026. doi: 10.3389/fmicb.2021.810026. eCollection 2021.
Nucleopolyhedroviruses (NPV, ) that infect lepidopteran pests have an established record as safe and effective biological insecticides. Here, we describe a new approach for the development of NPV-based insecticides. This technology takes advantage of the unique way in which these viruses are transmitted as collective infectious units, and the genotypic diversity present in natural virus populations. A ten-step procedure is described involving genotypic variant selection, mixing, coinfection and intraspecific coocclusion of variants within viral occlusion bodies. Using two examples, we demonstrate how this approach can be used to produce highly pathogenic virus preparations for pest control. As restricted host range limits the uptake of NPV-based insecticides, this technology has recently been adapted to produce custom-designed interspecific mixtures of viruses that can be applied to control complexes of lepidopteran pests on particular crops, as long as a shared host species is available for virus production. This approach to the development of NPV-based insecticides has the potential to be applied across a broad range of NPV-pest pathosystems.
感染鳞翅目害虫的核型多角体病毒（NPV）作为安全有效的生物杀虫剂已有既定的记录。在此，我们描述了一种开发基于NPV的杀虫剂的新方法。该技术利用了这些病毒作为集体感染单位进行传播的独特方式，以及自然病毒种群中存在的基因型多样性。描述了一个十步程序，包括基因型变体选择、混合、共感染以及病毒包涵体内变体的种内共包埋。通过两个例子，我们展示了这种方法如何用于生产用于害虫防治的高致病性病毒制剂。由于宿主范围有限限制了基于NPV的杀虫剂的应用，该技术最近已被调整用于生产定制设计的种间病毒混合物，只要有共同的宿主物种可用于病毒生产，这些混合物就可用于控制特定作物上的鳞翅目害虫复合体。这种开发基于NPV的杀虫剂的方法有可能应用于广泛的NPV-害虫病理系统。
