Zhang Y, Shafiq S A, Bader D
J Cell Biol. 1986 Apr;102(4):1480-4. doi: 10.1083/jcb.102.4.1480.
In the present study, a monoclonal antibody (McAb), ALD19, generated against myosin of slow tonic muscle, was shown to react with the heavy chain of ventricular myosin in the adult chicken heart. With this antibody, it was possible to detect a ventricular-specific myosin during myocardial differentiation and to show that the epitope recognized by ALD19 was present from the earliest stages of ventricular differentiation and maintained throughout development only in the ventricle. A second McAb, specific for atrial myosin heavy chain (MHC) (Gonzalez-Sanchez, A., and D. Bader, 1984, Dev. Biol., 103:151-158), was used as a control to detect an atrial-specific myosin in the caudal portion of the developing heart at Hamburger-Hamilton stage 15. It was found that the appearance of ventricular MHC predated the expression of atrial MHC by approximately 1 d in ovo and that specific MHCs were always differentially distributed. While a common primordial MHC may be present in the early heart, this study showed the tissue-specific expression of a ventricular MHC during the initial stages of heart development and its differential accumulation throughout development.
在本研究中，一种针对慢强直性肌肉肌球蛋白产生的单克隆抗体（McAb）ALD19，被证明可与成年鸡心脏中的心室肌球蛋白重链发生反应。利用该抗体，能够在心肌分化过程中检测到心室特异性肌球蛋白，并表明ALD19识别的表位从心室分化的最早阶段就已存在，且在整个发育过程中仅在心室中维持。第二种单克隆抗体，对心房肌球蛋白重链（MHC）具有特异性（Gonzalez-Sanchez，A.，和D. Bader，1984，《发育生物学》，103:151 - 158），用作对照以检测处于汉伯格 - 汉密尔顿第15阶段发育中心脏尾部的心房特异性肌球蛋白。结果发现，心室MHC的出现比心房MHC在卵内的表达提前约1天，并且特定的MHC总是呈差异分布。虽然早期心脏中可能存在共同的原始MHC，但本研究表明在心脏发育的初始阶段心室MHC的组织特异性表达及其在整个发育过程中的差异积累。
