Institute of Food Science and Technology, College of Life Science, Yangtze University, 266 Jingmi Road, Jingzhou, Hubei, China.
State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil &Water Pollution, Chengdu University of Technology, Chengdu, Sichuan, China.
Luminescence. 2022 Apr;37(4):665-671. doi: 10.1002/bio.4207. Epub 2022 Feb 17.
In this work, a highly sensitive biosensor for detecting cadmium ions (Cd ) was developed based on a Cd -specific DNA aptamer and a hybridization chain reaction (HCR). The Cd aptamer (named S0) was used to recognize Cd and trigger the HCR. Without Cd , S0 initiated the HCR to form long nicked dsDNA structures to quench the fluorescence. Then, Cd could bind with S0 to block HCR to recover fluorescence. This biosensor had high sensitivity with a detection limit of 0.36 nM and a linear range from 0 to 10 nM. Moreover, it showed a satisfactory selectivity and recovery rates.
在这项工作中，基于镉离子（Cd ）特异性 DNA 适体和杂交链式反应（HCR），开发了一种用于检测 Cd 的高灵敏度生物传感器。Cd 适体（命名为 S0）用于识别 Cd 并触发 HCR。没有 Cd 时，S0 引发 HCR 形成长的缺口 dsDNA 结构以猝灭荧光。然后，Cd 可以与 S0 结合以阻止 HCR 以恢复荧光。该生物传感器具有高灵敏度，检测限为 0.36 nM，线性范围为 0 至 10 nM。此外，它表现出令人满意的选择性和回收率。
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