Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400042, China.
Dalton Trans. 2022 Mar 1;51(9):3581-3589. doi: 10.1039/d1dt03624f.
All-inorganic CsPbBr perovskite quantum dots (QDs) are widely studied owing to their excellent optoelectronic properties; however, they are usually hydrophobic and unstable in water and thus their biomedical applications are seriously limited. In this study, stable and hydrophilic CsPbBr QDs functionalized with carboxyl groups (CsPbBr-COOH QDs) were prepared in one-pot with the aid of new ligands amino-poly(ethylene glycol)-carboxyl and perfluorooctyltriethoxylsilane. The aqueous solution of CsPbBr-COOH QDs maintained the initial fluorescence intensity after 8 days of storage; the free carboxyl groups on the surface of CsPbBr-COOH QDs were covalently conjugated with amino-terminal DNA to construct CsPbBr QDs-DNA probes for subsequent application. Then, a biosensing platform utilizing fluorescence resonance energy transfer between hydrophilic CsPbBr QDs-DNA and MoS nanosheets was developed for the sensitive and selective detection of the DNA with a low limit of detection of 51.9 pM and the identification of drug-resistant clinical strains. This study advances the preparation of hydrophilic carboxyl-functionalized CsPbBr QDs with enhanced stability and extends their application in biomolecule detection.
全无机 CsPbBr 钙钛矿量子点 (QDs) 因其优异的光电性能而被广泛研究；然而，它们通常在水中是疏水性和不稳定性的，因此它们的生物医学应用受到严重限制。在这项研究中，借助新配体氨基-聚（乙二醇）-羧基和全氟辛基三乙氧基硅烷，在一锅法中制备了稳定的亲水性羧基功能化的 CsPbBr QDs (CsPbBr-COOH QDs)。CsPbBr-COOH QDs 的水溶液在储存 8 天后仍保持初始荧光强度；CsPbBr-COOH QDs 表面的游离羧基与氨基末端 DNA 发生共价偶联，构建了 CsPbBr QDs-DNA 探针，用于后续应用。然后，开发了一个基于亲水 CsPbBr QDs-DNA 和 MoS 纳米片之间的荧光共振能量转移的生物传感平台，用于对 DNA 的灵敏和选择性检测，检测限低至 51.9 pM，并能识别耐药的临床菌株。这项研究推进了具有增强稳定性的亲水性羧基功能化 CsPbBr QDs 的制备，并扩展了它们在生物分子检测中的应用。
