利用活体成像技术研究斑马鱼早期心脏发育。

Using Live Imaging to Examine Early Cardiac Development in Zebrafish.

机构信息

Department of Biology, University of Mississippi, University, MS, USA.

出版信息

Methods Mol Biol. 2022;2438:133-145. doi: 10.1007/978-1-0716-2035-9_9.

DOI:10.1007/978-1-0716-2035-9_9

Abstract

Visualizing dynamic cellular behaviors using live imaging is critical to the study of cell movement and to the study of cellular and embryonic polarity. Similarly, live imaging can be vital to elucidating the pathology of genetic disorders and diseases. Model systems such as zebrafish, whose in vivo development is accessible to both the microscope and genetic manipulation, are particularly well-suited to the use of live imaging. Here we describe an overall approach to conducting live-imaging experiments with a specific emphasis on investigating cell movements during the early stages of heart development in zebrafish.

摘要

使用活体成像来可视化动态细胞行为对于研究细胞运动和细胞及胚胎极性至关重要。同样，活体成像是阐明遗传疾病和疾病病理学的关键。斑马鱼等模式系统，其体内发育既可以用显微镜观察，也可以进行遗传操作，特别适合进行活体成像。在这里，我们描述了一种进行活体成像实验的总体方法，特别强调了在斑马鱼心脏发育早期阶段研究细胞运动。