果蝇中肠的解剖、固定和免疫染色。

Dissection, Fixation, and Immunostaining of the Drosophila Midgut.

机构信息

The Gurdon Institute and the Department of Genetics, University of Cambridge, Cambridge, UK.

出版信息

Methods Mol Biol. 2022;2438:309-321. doi: 10.1007/978-1-0716-2035-9_20.

DOI:10.1007/978-1-0716-2035-9_20

Abstract

The Drosophila midgut is mainly composed of highly polarized epithelial cells called enterocytes that establish their apical-basal polarity in a fundamentally different way from other Drosophila epithelia. The roles of polarity factors in the midgut can be studied by generating clones of homozygous mutant cells in the background of wild-type tissue. In this chapter, we will introduce and discuss the procedures for producing positively marked mutant clones in the midgut and describe specific protocols for dissecting, fixing, and immunostaining this tissue.

摘要

果蝇中肠主要由高度极化的上皮细胞组成，称为肠细胞，它们在建立顶端-基底极性的方式上与其他果蝇上皮组织有根本的不同。通过在野生型组织背景下产生纯合突变细胞的克隆，可以研究极性因子在中肠中的作用。在本章中，我们将介绍和讨论在中肠中产生阳性标记突变克隆的程序，并描述解剖、固定和免疫染色这种组织的具体方案。