Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, P.R. China.
FASEB J. 2022 Mar;36(3):e22204. doi: 10.1096/fj.202101608R.
N6-methyladenosine is considered to be the most common and abundant internal chemical modification among the more than 150 identified chemical RNA modifications. It is involved in most biological processes and actively participates in the regulation of animal reproduction. However, the potential function of m A in the pituitaries of mammals is not yet clear. It is also unknown whether m A is involved in the secretion and regulation of FSH by GnRH, which in turn affects mammalian reproduction. In this study, rats were treated with gonadorelin to simulate physiological GnRH-mediated regulation of FSH synthesis and secretion, and m A-seq was used to analyze the differential m A modification of the rat pituitary after gonadorelin treatment. A whole-transcriptome map of m A in the rat pituitary gland before and after gonadorelin treatment was successfully created. A total of 6413 differential peaks were identified, of which 3764 m A peaks were upregulated and 2649 m A peaks were downregulated. Among the 709 differentially expressed genes, 250 genes were discovered with differential methylation modifications. Intriguingly, the altered m A peaks within mRNAs were enriched in steroid biosynthetic processes and responses to cAMP. The results of the study will lay a foundation for further exploration of the potential role of m A modification in the regulation of reproductive hormone secretion and provide a theoretical basis for the application of GnRH analogs in mammalian artificial reproduction.
N6-甲基腺苷被认为是在 150 多种已鉴定的化学 RNA 修饰中最常见和丰富的内部化学修饰。它参与了大多数生物过程，并积极参与了动物生殖的调控。然而，m A 在哺乳动物垂体中的潜在功能尚不清楚。也不知道 m A 是否参与 GnRH 介导的 FSH 的分泌和调节，而 FSH 的分泌和调节又反过来影响哺乳动物的生殖。在这项研究中，用促性腺激素释放激素类似物处理大鼠，模拟生理促性腺激素释放激素介导的 FSH 合成和分泌的调节，并用 m A-seq 分析促性腺激素释放激素类似物处理后大鼠垂体中 m A 的差异修饰。成功创建了促性腺激素释放激素类似物处理前后大鼠垂体 m A 的全转录组图谱。共鉴定出 6413 个差异峰，其中 3764 个 m A 峰上调，2649 个 m A 峰下调。在 709 个差异表达基因中，发现有 250 个基因具有差异甲基化修饰。有趣的是，mRNA 内改变的 m A 峰富集在甾体生物合成过程和对 cAMP 的反应中。该研究的结果将为进一步探索 m A 修饰在调节生殖激素分泌中的潜在作用奠定基础，并为 GnRH 类似物在哺乳动物人工繁殖中的应用提供理论依据。
