Proteome and transcriptome explore the mechanism of Salvia miltiorrhiza polysaccharides to relieve florfenicol-induced kidney injury in broilers.

This experiment explored the mechanism of Salvia miltiorrhiza polysaccharides (SMPs) on florfenicol (FFC)-induced kidney injury in broilers. Ninety healthy 1-day-old Arbor Acres broilers were randomly divided into 3 groups with 6 replicates in each group and 5 chickens in each replicate. The three groups included control group, model group (0.15 g/L FFC), and SMPs group (0.15 g/L FFC + 5.00 g/L SMPs). After 5 days of experimental period, blood was collected, and kidney tissues were extracted. Renal injury was evaluated by serum biochemical indicators and pathological sections. Renal oxidative stress indexes were detected; transcriptomics and proteomics were used to comprehensively analyze the effects of SMPs on broiler kidney injury. The results showed that the model group inhibited average day gain (P < 0.01) and significantly adjusted blood urea nitrogen (BUN), uric acid (UA), and creatinine (Cr) (P < 0.01 or P < 0.05). The histological observation of the kidneys in the model group showed abnormal morphology, and the oxidative stress parameters showed that FFC induced oxidative stress in the kidneys. Comprehensive transcriptome proteomic analysis data showed phosphoribose pyrophosphate synthase 2 (PRPS2), cytochrome 2AC1 (CYP2AC1), cytochrome 2D6 (CYP2D6), glutathione transferase (GST), and sulfotransferase 1B (SULT1B) expression levels changed. It is worth noting that our data showed that supplementation of 5.00 g/L SMPs in drinking water reversed the changes in BUN, Cr, and daily weight gain (P < 0.05) and relieved the abnormal kidney morphology caused by FFC. After SMPs processing, it improved the detoxification process of drug-metabolizing enzymes and improved the oxidative stress state induced by FFC. Therefore, SMPs reduced the nephrotoxicity caused by FFC by promoting drug-metabolizing enzymes and alleviating oxidative stress in the kidneys.