de Jong J G, Dekker A W, van Kessel K P, Sixma J J
Leuk Res. 1986;10(4):437-43. doi: 10.1016/0145-2126(86)90074-3.
Using membranes isolated from leukemic myeloblasts as immunogen, two granulocyte specific monoclonal antibodies 38D2 and 41D2 were developed. The antibodies are positive in membrane immunofluorescence with granulocytes and bands; they give no reaction with monocytes, platelets, leukemic myeloblasts, lymphoblasts, myeloid cell lines, and immature myeloid cells from bone marrow. Both monoclonal antibodies precipitate the same membrane glycoprotein (MW: 70-78 kd, pI: 6-7), which corresponds to a membrane glycoprotein characteristic for granulocytes, as concluded from two-dimensional polyacrylamide gelelectrophoresis patterns obtained from surface labelled myeloid cells. The monoclonal antibodies did not influence the granulocyte functions tested: phagocytosis of opsonized bacteria, chemiluminiscence after stimulation with various stimuli, and chemotaxis.
以从白血病成髓细胞中分离出的膜作为免疫原，研制出了两种粒细胞特异性单克隆抗体38D2和41D2。这些抗体在粒细胞和带状核细胞的膜免疫荧光检测中呈阳性；它们与单核细胞、血小板、白血病成髓细胞、淋巴母细胞、髓系细胞系以及来自骨髓的未成熟髓系细胞均无反应。两种单克隆抗体沉淀出相同的膜糖蛋白（分子量：70 - 78千道尔顿，等电点：6 - 7），从表面标记的髓系细胞获得的二维聚丙烯酰胺凝胶电泳图谱来看，该糖蛋白对应于粒细胞特有的一种膜糖蛋白。单克隆抗体不影响所检测的粒细胞功能：调理素化细菌的吞噬作用、各种刺激物刺激后的化学发光以及趋化性。
