Skubitz K M, August J T
Arch Biochem Biophys. 1985 Apr;238(1):263-71. doi: 10.1016/0003-9861(85)90164-x.
Major surface-iodinated proteins of Mr 105,000 and 145,000 of normal human neutrophils are immunoprecipitated by a number of monoclonal antibodies (AHN-1 to AHN-6), which react specifically with granulocytes among peripheral blood cells and selectively inhibit phagocytosis. These proteins, and an Mr 60,000 component, were purified by monoclonal antibody affinity chromatography, molecular sieve chromatography, and preparative polyacrylamide gel electrophoresis. Each of the three purified proteins was immunoprecipitated by all six antibodies. Nevertheless, tryptic peptide maps of the three proteins indicated that each was a distinct component. AHN-1 to AHN-6 also bound to glycolipid fractions of human neutrophils, and the binding of each antibody to human neutrophils was blocked by the carbohydrate sequences, lacto-N-fucopentaose III. The data indicate that a predominant antigenic determinant of human neutrophils is lacto-N-fucopentaose III, or related carbohydrates, present on three distinct proteins as well as glycolipids. At least one of these molecules appears to be involved in the process of phagocytosis.
正常人类中性粒细胞中分子量为105,000和145,000的主要表面碘化蛋白可被多种单克隆抗体（AHN - 1至AHN - 6）免疫沉淀，这些抗体在外周血细胞中与粒细胞特异性反应，并选择性抑制吞噬作用。这些蛋白质以及一个分子量为60,000的成分通过单克隆抗体亲和层析、分子筛层析和制备性聚丙烯酰胺凝胶电泳进行纯化。三种纯化蛋白中的每一种都能被所有六种抗体免疫沉淀。然而，这三种蛋白质的胰蛋白酶肽图谱表明它们各自是不同的成分。AHN - 1至AHN - 6也与人类中性粒细胞的糖脂部分结合，并且每种抗体与人类中性粒细胞的结合都被碳水化合物序列乳糖 - N - 岩藻五糖III阻断。数据表明，人类中性粒细胞的主要抗原决定簇是乳糖 - N - 岩藻五糖III或相关碳水化合物，它们存在于三种不同的蛋白质以及糖脂上。这些分子中至少有一种似乎参与了吞噬过程。
