卡波氏肉瘤相关疱疹病毒（KSHV/HHV8）的全基因组测序显示存在两个非洲谱系的证据。
Whole-genome sequencing of Kaposi sarcoma-associated herpesvirus (KSHV/HHV8) reveals evidence for two African lineages.
机构信息
Lineberger Comprehensive Cancer Center, School of Medicine, Department of Immunology and Microbiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Lineberger Comprehensive Cancer Center, School of Medicine, Department of Immunology and Microbiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
出版信息
Virology. 2022 Mar;568:101-114. doi: 10.1016/j.virol.2022.01.005. Epub 2022 Feb 2.
Kaposi sarcoma (KS)-associated herpesvirus (KSHV/HHV-8) was first sequenced from the body cavity (BC) lymphoma cell line, BC-1, in 1996. Few other KSHV genomes have been reported. Our knowledge of sequence variation for this virus remains spotty. This study reports additional genomes from historical US patient samples and from African KS biopsies. It describes an assay that spans regions of the virus that cannot be covered by short read sequencing. These include the terminal repeats, the LANA repeats, and the origins of replication. A phylogenetic analysis, based on 107 genomes, identified three distinct clades; one containing isolates from USA/Europe/Japan collected in the 1990s and two of Sub-Saharan Africa isolates collected since 2010. This analysis indicates that the KSHV strains circulating today differ from the isolates collected at the height of the AIDS epidemic. This analysis helps experimental designs and potential vaccine studies.
卡波西肉瘤（KS）相关疱疹病毒（KSHV/HHV-8）于 1996 年首次从体腔（BC）淋巴瘤细胞系 BC-1 中测序。此后报道了少数其他 KSHV 基因组。我们对该病毒序列变异的了解仍然很零散。本研究报告了来自美国历史患者样本和非洲 KS 活检的其他基因组。它描述了一种跨越病毒无法通过短读测序覆盖的区域的检测方法。这些区域包括末端重复序列、LANA 重复序列和复制起点。基于 107 个基因组的系统发育分析确定了三个不同的分支；一个包含来自美国/欧洲/日本的分离株，这些分离株是在 20 世纪 90 年代收集的，另外两个分支包含 2010 年以来在撒哈拉以南非洲收集的分离株。该分析表明，目前循环的 KSHV 株与艾滋病流行高峰期采集的分离株不同。该分析有助于实验设计和潜在的疫苗研究。
Zotero 插件