Staines W A, Yamamoto T, Daddona P E, Nagy J I
Neurosci Lett. 1986 Sep 25;70(1):1-5. doi: 10.1016/0304-3940(86)90427-1.
Neurons of the posterior hypothalamic magnocellular nucleus (PHMN) in the rat are immunoreactive for adenosine deaminase (ADA) and cells coextensive with this nucleus show immunoreactivity for serotonin after pretreatment with 5-hydroxytryptophan (5-HTP). The degree to which ADA coexists in cells having 5-HTP uptake capability was analyzed by a new double immunohistofluorescence procedure employing diethylaminocoumarin (DAMC). It was determined that 5-HTP accumulating cells of the PHMN represent a subpopulation of those immunoreactive for ADA. These results add further to the neurochemical characterization of this globally projecting hypothalamic cell group and point out the utility of DAMC as a new immunohistofluorescence marker.
大鼠下丘脑后大细胞核对腺苷脱氨酶（ADA）呈免疫反应性，在用5-羟色氨酸（5-HTP）预处理后，与该核共延的细胞对血清素呈免疫反应性。采用二乙氨基香豆素（DAMC）的一种新的双重免疫荧光方法分析了ADA在具有5-HTP摄取能力的细胞中的共存程度。结果表明，PHMN中积累5-HTP的细胞代表了对ADA呈免疫反应性的细胞亚群。这些结果进一步补充了这个具有整体投射功能的下丘脑细胞群的神经化学特征，并指出了DAMC作为一种新的免疫荧光标记物的实用性。
