Hendricks L D, Wood D E, Hajduk M E
Parasitology. 1978 Jun;76(3):309-16. doi: 10.1017/s0031182000048186.
The successful cultivation of a variety of haemoflagellates in three different liquid media is reported. These media include medium 199, Grace's insect tissue-culture medium and Schneider's drosophila medium, each in combination with 30% (v/v) foetal calf serum. These media were used to cultivate Old and New World species of visceral and cutaneous human Leishmania, as well as Leishmania species isolated from sandflies, rodents, and reptiles. Four strains of Trypanosoma cruzi, an isolate of T. R-angeli and and an isolate of T. lewisi have also been cultivated in these media. One or more of these media have been used to cultivate 121 strains of haemoflagellates, including at least 14 different species (11 Leishmania and 3 Trypanosoma) and many geographic isolates or strains. The Leishmania include L. braziliensis, L. peruviana, L. mexicana, L. tropica, L. donovani, L. chagasi, L. enriettii, L. hertigi, L. hoogstraali, L. adleri, and L. agamae. Using the Schneider's based medium, we have obtained primary isolates of both cutaneous and visceral Leishmania of man and of experimentally infected laboratory rodents and canines. Freeze-dried preparations of the Schneider's based medium that were reconsituted with distilled water after 24 months of storage at ambient temperature have proven to be suitable cultivation media. This feature makes the media valuable field tools. The various species of human Leishmania cultivated in these media have in our experience demonstrated no differences in growth rate, viability after liquid nitrogen preservation, or infectivity for laboratory animals and tissue-culture cells compared with promastigotes derived from blood-agar cultivation.
据报道，多种血鞭毛虫已成功在三种不同的液体培养基中培养。这些培养基包括199培养基、格雷斯昆虫组织培养基和施奈德果蝇培养基，每种培养基均与30%（v/v）胎牛血清混合使用。这些培养基用于培养人类内脏和皮肤利什曼原虫的新旧世界物种，以及从白蛉、啮齿动物和爬行动物中分离出的利什曼原虫物种。四种克氏锥虫菌株、一种R-安氏锥虫分离株和一种刘易斯锥虫分离株也已在这些培养基中培养。这些培养基中的一种或多种已用于培养121株血鞭毛虫，包括至少14个不同物种（11种利什曼原虫和3种锥虫）以及许多地理分离株或菌株。利什曼原虫包括巴西利什曼原虫、秘鲁利什曼原虫、墨西哥利什曼原虫、热带利什曼原虫、杜氏利什曼原虫、恰加斯利什曼原虫、恩氏利什曼原虫、赫氏利什曼原虫、霍氏利什曼原虫、阿氏利什曼原虫和沙蜥利什曼原虫。使用基于施奈德培养基，我们获得了人类皮肤和内脏利什曼原虫以及实验感染的实验室啮齿动物和犬类的原始分离株。在室温下储存24个月后用蒸馏水重新配制的基于施奈德培养基的冻干制剂已被证明是合适的培养基。这一特性使这些培养基成为有价值的现场工具。根据我们的经验，在这些培养基中培养的各种人类利什曼原虫物种与从血琼脂培养中获得的前鞭毛体相比，在生长速率、液氮保存后的活力或对实验动物和组织培养细胞的感染性方面没有差异。
