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酶联免疫吸附测定法测定大嘴乌鸦尿中中催产素。

Measurement of urinary mesotocin in large-billed crows by enzyme-linked immunosorbent assay.

机构信息

Department of Psychology, Keio University, Tokyo, Japan.

School of Veterinary Medicine, Azabu University, Kanagawa, Japan.

出版信息

J Vet Med Sci. 2022 Apr 13;84(4):520-524. doi: 10.1292/jvms.21-0635. Epub 2022 Feb 14.

DOI:10.1292/jvms.21-0635
PMID:35153252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9096038/
Abstract

Mesotocin (MT) is an avian homologue of oxytocin (OT). Behavioral pharmacological studies in birds have suggested the involvement of MT in socially affiliative behavior. However, investigations of peripheral MT levels associated with social behavior are lacking because non-invasive methods to measure surrogate plasma MT have yet to be established. This study aimed to measure urinary MT in crows using a commercially available OT enzyme-linked immunosorbent assay kit. Urine samples were collected after intravenous injection of MT and centrifuged to separate urine and fecal components. We found that urinary MT was significantly elevated 15-30 min after MT injection. These results validate our method for the use of urine samples for the measurement of peripheral MT levels in crows.

摘要

中松催产素 (MT) 是催产素 (OT) 的鸟类同源物。鸟类的行为药理学研究表明，MT 参与了社交亲和行为。然而，由于尚未建立测量替代血浆 MT 的非侵入性方法，因此缺乏与社交行为相关的外周 MT 水平的研究。本研究旨在使用市售的 OT 酶联免疫吸附测定试剂盒测量乌鸦的尿 MT。静脉注射 MT 后采集尿液样本并离心以分离尿液和粪便成分。我们发现，MT 注射后 15-30 分钟，尿 MT 水平显著升高。这些结果验证了我们使用尿液样本测量乌鸦外周 MT 水平的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d0/9096038/03fa387d2c8f/jvms-84-520-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d0/9096038/17ef188dc9e2/jvms-84-520-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d0/9096038/258997dc1944/jvms-84-520-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d0/9096038/03fa387d2c8f/jvms-84-520-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d0/9096038/17ef188dc9e2/jvms-84-520-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d0/9096038/258997dc1944/jvms-84-520-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d0/9096038/03fa387d2c8f/jvms-84-520-g003.jpg

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