Murata Kaori, Nagasawa Miho, Onaka Tatsushi, Takeyama Ken-Ichi, Kikusui Takefumi
School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan.
Center for Human and Animal Symbiosis Science, Azabu University, Kanagawa 252-5201, Japan.
J Vet Med Sci. 2021 Apr 3;83(3):478-481. doi: 10.1292/jvms.20-0723. Epub 2021 Jan 20.
The biological and psychological significance of oxytocin is increasingly recognized; however, reliable assays of oxytocin in biological samples have not been developed. We raised a new oxytocin polyclonal rabbit antibody against synthetic oxytocin. The affinity of antibodies to oxytocin was examined by a radio-immunoassay and compared with that of a previously validated antibody. One antibody showed affinity for oxytocin in the radio-immunoassay. We developed a solid-phase ELISA for oxytocin using this antibody and compared it with existing methods. The newly developed ELISA showed comparable results using urine samples but not using serum samples. These results indicate that the new ELISA is useful for urinary oxytocin; further modifications, such as different extraction methods, are needed for its application to serum oxytocin.
催产素的生物学和心理学意义日益得到认可；然而，尚未开发出可靠的生物样品中催产素检测方法。我们制备了一种针对合成催产素的新型兔抗催产素多克隆抗体。通过放射免疫测定法检测抗体与催产素的亲和力，并与先前验证过的抗体进行比较。一种抗体在放射免疫测定中显示出对催产素的亲和力。我们使用该抗体开发了一种用于催产素的固相酶联免疫吸附测定法（ELISA），并将其与现有方法进行比较。新开发的ELISA在使用尿液样本时显示出可比的结果，但在使用血清样本时则不然。这些结果表明，新的ELISA对尿液中的催产素检测有用；若要将其应用于血清催产素检测，还需要进一步改进，如采用不同的提取方法。
