Zheng Lining, Gu Xuehu, Sun Liangpeng, Dong Meiqi, Gao Ao, Han Zhe, Pan Hongyu, Zhang Hao
College of Plant Protection, Jilin Agricultural University, Changchu 130118, China.
College of Plant Sciences, Jilin University, Changchun 130062, China.
J Fungi (Basel). 2023 Apr 28;9(5):526. doi: 10.3390/jof9050526.
D50, a biocontrol strain, is used to prevent and treat the fungal plant pathogen . D50's biofilms can affect its colonization; thus, the effects of different metal ions and culture conditions on biofilm formation were determined in this study. The results of medium optimization showed that Ca had the best ability to promote biofilm formation. The optimal medium composition for the formation of biofilms contained tryptone (10 g/L), CaCl (5.14 g/L), and yeast extract (5.0 g/L), and the optimal fermentation conditions included pH 7, a temperature of 31.4 °C, and a culture time of 51.8 h. We found that the antifungal activity and abilities to form biofilms and colonize roots were improved after optimization. In addition, the levels of expression of the genes , , , and were up-regulated by 37.56-, 2.87-, 12.46-, and 6.22-fold, respectively. The soil enzymatic activities which related biocontrol-related enzymes were the highest when the soil was treated by strain D50 after optimization. In vivo biocontrol assays indicated that the biocontrol effect of strain D50 after optimization was improved.
生防菌株D50用于防治植物真菌病原体。D50的生物膜会影响其定殖；因此，本研究测定了不同金属离子和培养条件对生物膜形成的影响。培养基优化结果表明，钙促进生物膜形成的能力最强。生物膜形成的最佳培养基组成包含胰蛋白胨（10 g/L）、氯化钙（5.14 g/L）和酵母提取物（5.0 g/L），最佳发酵条件包括pH 7、温度31.4℃和培养时间51.8小时。我们发现优化后抗真菌活性以及形成生物膜和定殖于根部的能力均得到提高。此外，相关基因的表达水平分别上调了37.56倍、2.87倍、12.46倍和6.22倍。优化后用菌株D50处理土壤时，与生物防治相关酶有关的土壤酶活性最高。体内生物防治试验表明，优化后菌株D50的生物防治效果得到提高。
