Yu Qihong, Liu Xinru, Huang Haojie, Zheng Xingfeng, Pan Xue, Fang Junwei, Meng Liyuan, Zhou Chunhua, Zhang Xiaocui, Li Zhaoshen, Zou Duowu
Digestive Department, Changhai Hospital, Shanghai, China.
Institute of Human Phenotypes, Fudan University, Shanghai, China.
Therap Adv Gastroenterol. 2019 Nov 7;12:1756284819886425. doi: 10.1177/1756284819886425. eCollection 2019.
Irritable bowel syndrome (IBS) is a common gastrointestinal disorder without obvious structural abnormalities or consistent associated biomarkers, making its diagnosis difficult. In the present study, we used a urine-based metabolomics approach to identify IBS biomarkers.
We used an ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) on urine samples from patients suffering from IBS and healthy controls. Data were coupled for multivariate statistical analysis methods.
We selected 30 differential metabolites associated with IBS and found steroid hormone biosynthesis and histidine metabolism alterations in patients with IBS that may be involved in the pathogenesis of the disease. In addition, we identified a panel of five metabolite markers composed of cortisone, citric acid, tiglylcarnitine, N6,-N6,-N6-trimethyl-L-lysine and L-histidine that could be used to discriminate between patients and healthy controls and may be appropriate as IBS diagnosis biomarkers.
Our findings indicate that metabolomics combined with pattern recognition can be useful to identify disease diagnostic IBS markers.
ChiCTR1800020072.
肠易激综合征（IBS）是一种常见的胃肠道疾病，无明显结构异常或一致的相关生物标志物，导致其诊断困难。在本研究中，我们采用基于尿液的代谢组学方法来识别IBS生物标志物。
我们对IBS患者和健康对照的尿液样本使用超高效液相色谱/四极杆飞行时间质谱（UPLC-QTOF-MS）。数据采用多变量统计分析方法进行处理。
我们筛选出30种与IBS相关的差异代谢物，发现IBS患者存在类固醇激素生物合成和组氨酸代谢改变，这可能参与了该疾病的发病机制。此外，我们鉴定出一组由可的松、柠檬酸、 tiglylcarnitine、N6,-N6,-N6-三甲基-L-赖氨酸和L-组氨酸组成的五种代谢物标志物，可用于区分患者和健康对照，可能适合作为IBS诊断生物标志物。
我们的研究结果表明，代谢组学结合模式识别有助于识别IBS疾病诊断标志物。
ChiCTR1800020072。
