Department of Interdisciplinary Research in Science, Alexandru Ioan Cuza University of Iași, Carol I Avenue, No. 11, Iași, Romania.
Department of Research, Faculty of Biology, Alexandru Ioan Cuza University of Iași, Carol I Avenue, 20A, Iași, Romania.
Oxid Med Cell Longev. 2020 May 23;2020:4690713. doi: 10.1155/2020/4690713. eCollection 2020.
According to the latest gastrointestinal disorders diagnostic criteria (ROME IV), the irritable bowel syndrome (IBS) is mainly characterized by the presence of abdominal pain and changes in intestinal transit. However, both sleep impairments and oxidative status changes (in patients' sera, mucosal level, and other body fluids) were reported IBS. Thus, in this study, we aimed to evaluate several aspects regarding the oxidative stress status in patients' tears as well as sleep disturbances by comparison with the intensity of IBS symptoms, as assessed by the visual analogue scale for irritable bowel syndrome (VAS-IBS). Ten IBS patients and fourteen healthy sex- and age-matched volunteers were recruited from the Oftaprof Ophthalmological Clinic (Iași, Romania). Visual analogue scale for irritable bowel syndrome and the Pittsburgh Sleep Quality Index (PSQI) questionnaires were administered to all the patients. Tear samples were collected using the Schirmer test procedure and were subjected to biochemical analysis-superoxide dismutase and glutathione peroxidase activities, malondialdehyde, and total soluble proteins levels were determined. Standard statistical analysis was applied. We found significant differences in oxidative stress marker dynamics in IBS patients as compared to healthy age- and sex-matched controls: increased superoxide dismutase activity ( = 0.02), increased malondialdehyde ( = 0.007), and total soluble proteins levels ( = 0.019). We found no significant differences in tear glutathione peroxidase activity in IBS patients as compared to healthy age- and sex-matched controls ( = 0.55). Furthermore, we observed that the oxidative stress tear markers are correlated with gastrointestinal symptoms severity (as evaluated by VAS-IBS) but not correlated to the sleep quality index and items (as evaluated by PSQI), with significant differences according to patient sex and IBS subtype stratification. In this way, this study brings additional evidence of the oxidative stress role in IBS pathology alongside the evaluation of tear fluid molecular dynamics in IBS for the first time in our best knowledge.
根据最新的胃肠疾病诊断标准（ROME IV），肠易激综合征（IBS）主要表现为腹痛和肠道转运改变。然而，有报道称，IBS 患者存在睡眠障碍和氧化状态改变（在患者血清、黏膜水平和其他体液中）。因此，在这项研究中，我们旨在通过比较视觉模拟量表评估 IBS 症状强度（VAS-IBS），评估患者泪液中的氧化应激状态以及睡眠障碍。从罗马尼亚雅西的 Oftaprof 眼科诊所招募了 10 名 IBS 患者和 14 名性别和年龄匹配的健康志愿者。对所有患者进行了 VAS-IBS 和匹兹堡睡眠质量指数（PSQI）问卷评估。使用 Schirmer 测试程序收集泪液样本，并进行生化分析-超氧化物歧化酶和谷胱甘肽过氧化物酶活性、丙二醛和总可溶性蛋白水平的测定。应用标准统计分析。我们发现 IBS 患者与健康年龄和性别匹配的对照组相比，氧化应激标志物动态存在显著差异：超氧化物歧化酶活性增加（ = 0.02）、丙二醛增加（ = 0.007）和总可溶性蛋白水平增加（ = 0.019）。我们发现 IBS 患者的泪液谷胱甘肽过氧化物酶活性与健康年龄和性别匹配的对照组无显著差异（ = 0.55）。此外，我们观察到氧化应激泪液标志物与胃肠道症状严重程度（如 VAS-IBS 评估）相关，但与睡眠质量指数和项目（如 PSQI 评估）不相关，根据患者性别和 IBS 亚型分层存在显著差异。通过这种方式，这项研究为 IBS 病理学中的氧化应激作用提供了额外的证据，同时也是首次在我们的最佳知识范围内评估 IBS 患者的泪液分子动力学。
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