Xing Xiaojing, Xu Tonghong, Liu Bin, Guo Qianxue
Department of Hematology and Breast Cancer, Cancer Hospital of China Medical University, Shenyang 110042, Liaoning, P.R.China.
Department of Hematology and Breast Cancer, Liaoning Cancer Hospital & Institute, Shenyang 110042, Liaoning, P.R.China.
J Cancer. 2022 Jan 1;13(3):784-792. doi: 10.7150/jca.60521. eCollection 2022.
It has been verified that long noncoding RNAs (lncRNAs) may participate in the pathogenesis of various human diseases. This study aims to investigate the roles of lncRNA SNHG5 in diffuse large B cell lymphoma (DLBC), especially the impacts of lncRNA SNHG5 on proliferation and migration of human diffuse large B cell lymphoma cells and the related mechanism. qRT-PCR analysis was carried out to examine the expression pattern of SNHG5 in DLBC tissue and adjacent normal tissue. The effect of SNHG5 on the proliferation, apoptosis, migration, and invasion of DLBC cells was detected by MTT, flow cytometry analysis, wound healing assay and transwell assay. The correlation between SNHG5, miR-181-5p and XIAP were certified by bioinformatics analysis, and dual-luciferase reporter assay. Furthermore, rescue assays were performed to analyze the effects of SNHG5-miR-181-5p-XIAP axis on the biological behaviors of diffuse large B cell lymphoma cells. Finally, the effects of SNHG5 axis on the growth of DLBC tumor was examined by analysis. SNHG5 was significantly upregulated in diffuse large B cell lymphoma tissues. Knockdown of SNHG5 inhibited the proliferation, migration, and invasion of diffuse large B cell lymphoma cells and . LncRNA SNHG5 acted as a ceRNA through binding with miR-181-5p in DLBC cells. LncRNA SNHG5 may promote proliferation and migration of diffuse large B cell lymphoma cells via targeting miR-181-5p/XIAP.
现已证实，长链非编码RNA（lncRNA）可能参与多种人类疾病的发病机制。本研究旨在探讨lncRNA SNHG5在弥漫性大B细胞淋巴瘤（DLBC）中的作用，尤其是lncRNA SNHG5对人弥漫性大B细胞淋巴瘤细胞增殖和迁移的影响及其相关机制。采用qRT-PCR分析检测SNHG5在DLBC组织及相邻正常组织中的表达模式。通过MTT、流式细胞术分析、伤口愈合试验和Transwell试验检测SNHG5对DLBC细胞增殖、凋亡、迁移和侵袭的影响。通过生物信息学分析和双荧光素酶报告基因试验验证SNHG5、miR-181-5p和XIAP之间的相关性。此外，进行拯救试验以分析SNHG5-miR-181-5p-XIAP轴对弥漫性大B细胞淋巴瘤细胞生物学行为的影响。最后，通过分析检测SNHG5轴对DLBC肿瘤生长的影响。SNHG5在弥漫性大B细胞淋巴瘤组织中显著上调。敲低SNHG5可抑制弥漫性大B细胞淋巴瘤细胞的增殖、迁移和侵袭。在DLBC细胞中，lncRNA SNHG5通过与miR-181-5p结合充当竞争性内源RNA（ceRNA）。lncRNA SNHG5可能通过靶向miR-181-5p/XIAP促进弥漫性大B细胞淋巴瘤细胞的增殖和迁移。
