Liang Yicheng, Lei Yangyang, Liang Mei, Du Minjun, Liu Zixu, Li Xingkai, Meng Xiangzhi, Zhou Boxuan, Gao Yushun
Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Department of Interventional Radiology, Shanghai Institute of Medical Imaging, Shanghai, China.
Front Oncol. 2022 Jan 27;11:781344. doi: 10.3389/fonc.2021.781344. eCollection 2021.
Glycogen branching enzyme (GBE1) is a critical gene that participates in regulating glycogen metabolism. However, the correlations between GBE1 expression and the prognosis and tumor-associated macrophages in lung adenocarcinoma (LUAD) also remain unclear. Herein, we firstly analyzed the expression level of GBE1 in LUAD tissues and adjacent lung tissues The Cancer Genome Atlas (TCGA) database. The effect of GBE1 on prognosis was estimated by utilizing TCGA database and the PrognoScan database. The relationships between the clinical characteristics and GBE1 expression were evaluated TCGA database. We then investigated the relationships between GBE1 and infiltration of immune cells in LUAD by utilizing the CIBERSORT algorithm and Tumor Immune Estimation Resource (TIMER) database. In addition, we used a tissue microarray (TMA) containing 92 LUAD tissues and 88 adjacent lung tissues with immunohistochemistry staining to verify the association between GBE1 expression and clinical characteristics, as well as the immune cell infiltrations. We found the expression level of GBE1 was significantly higher in LUAD tissues. High expression of GBE1 was associated with poorer overall survival (OS) in LUAD. In addition, high expression of GBE1 was correlated with advanced T classification, N classification, M classification, TNM stage, and lower grade. Moreover, GBE1 was positively correlated with infiltrating levels of CD163 tumor-associated macrophages in LUAD. In conclusion, the expression of GBE1 is associated with the prognosis and CD163 tumor-associated macrophage infiltration in LUAD, suggesting that it has potential to be prognostic and immunological biomarkers in LUAD.
糖原分支酶（GBE1）是参与调节糖原代谢的关键基因。然而，GBE1表达与肺腺癌（LUAD）的预后及肿瘤相关巨噬细胞之间的相关性仍不清楚。在此，我们首先利用癌症基因组图谱（TCGA）数据库分析了GBE1在LUAD组织和邻近肺组织中的表达水平。通过使用TCGA数据库和PrognoScan数据库评估GBE1对预后的影响。利用TCGA数据库评估临床特征与GBE1表达之间的关系。然后，我们利用CIBERSORT算法和肿瘤免疫评估资源（TIMER）数据库研究了GBE1与LUAD中免疫细胞浸润之间的关系。此外，我们使用包含92个LUAD组织和88个邻近肺组织的组织芯片（TMA）进行免疫组化染色，以验证GBE1表达与临床特征以及免疫细胞浸润之间的关联。我们发现GBE1在LUAD组织中的表达水平显著更高。GBE1的高表达与LUAD患者较差的总生存期（OS）相关。此外，GBE1的高表达与晚期T分级、N分级、M分级、TNM分期以及较低的分级相关。而且，GBE1与LUAD中CD163肿瘤相关巨噬细胞的浸润水平呈正相关。总之，GBE1的表达与LUAD的预后及CD163肿瘤相关巨噬细胞浸润有关，表明它有可能成为LUAD的预后和免疫生物标志物。
