Ma Xiaofei, Øvrebø Jan Inge, Thompson Eric M
College of Life Sciences, Northwest Normal University, Lanzhou, China.
Sars International Centre, University of Bergen, Bergen, Norway.
Front Cell Dev Biol. 2022 Jan 28;9:770939. doi: 10.3389/fcell.2021.770939. eCollection 2021.
The active site of the essential CDK1 kinase is generated by core structural elements, among which the PSTAIRE motif in the critical αC-helix, is universally conserved in the single CDK1 ortholog of all metazoans. We report serial duplications in the chordate, . Paralog diversifications in the PSTAIRE, activation loop substrate binding platform, ATP entrance site, hinge region, and main Cyclin binding interface, have undergone positive selection to subdivide ancestral CDK1 functions along the S-M phase cell cycle axis. Apparent coevolution of an exclusive CDK1d:Cyclin Ba/b pairing is required for oogenic meiosis and early embryogenesis, a period during which, unusually, CDK1d, rather than Cyclin Ba/b levels, oscillate, to drive very rapid cell cycles. Strikingly, the modified PSTAIRE of odCDK1d shows convergence over great evolutionary distance with plant CDKB, and in both cases, these variants exhibit increased specialization to M-phase.
必需的CDK1激酶的活性位点由核心结构元件产生，其中关键αC螺旋中的PSTAIRE基序在所有后生动物的单一CDK1直系同源物中普遍保守。我们报道了脊索动物中的系列重复。PSTAIRE、激活环底物结合平台、ATP入口位点、铰链区和主要细胞周期蛋白结合界面中的旁系同源物多样化经历了正选择，以沿着S-M期细胞周期轴细分祖先CDK1的功能。卵子发生减数分裂和早期胚胎发生需要独特的CDK1d:细胞周期蛋白Ba/b配对的明显共同进化，在此期间，不同寻常的是，CDK1d而非细胞周期蛋白Ba/b水平振荡，以驱动非常快速的细胞周期。引人注目的是，odCDK1d的修饰PSTAIRE在很大的进化距离上与植物CDKB趋同，并且在这两种情况下，这些变体对M期表现出增加的特异性。
