Feng Haiyang, Thompson Eric M
Institute of Biological Sciences, Jinzhou Medical University, Jinzhou, China.
Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen, Norway.
Front Cell Dev Biol. 2023 Dec 7;11:1323378. doi: 10.3389/fcell.2023.1323378. eCollection 2023.
A single Aurora kinase found in non-vertebrate deuterostomes is assumed to represent the ancestor of vertebrate Auroras A/B/C. However, the tunicate , a member of the sister group to vertebrates, possesses two Aurora kinases (Aurora1 and Aurora2) that are expressed in proliferative cells and reproductive organs. Previously, we have shown that Aurora kinases relocate from organizing centers to meiotic nuclei and were enriched on centromeric regions as meiosis proceeds to metaphase I. Here, we assessed their respective functions in oogenic meiosis using dsRNA interferences. We found that Aurora1 (Aur1) was involved in meiotic spindle organization and chromosome congression, probably through the regulation of microtubule dynamics, whereas Aurora2 (Aur2) was crucial for chromosome condensation and meiotic spindle assembly. kinase assays showed that Aur1 and Aur2 had comparable levels of kinase activities. Using yeast two-hybrid library screening, we identified a few novel interaction proteins for Aur1, including c-Jun-amino-terminal kinase-interacting protein 4, cohesin loader Scc2, and mitochondrial carrier homolog 2, suggesting that Aur1 may have an altered interaction network and participate in the regulation of microtubule motors and cohesin complexes in .
在非脊椎后口动物中发现的单一极光激酶被认为是脊椎动物极光激酶A/B/C的祖先。然而，作为脊椎动物姐妹群成员的被囊动物拥有两种极光激酶（极光激酶1和极光激酶2），它们在增殖细胞和生殖器官中表达。此前，我们已经表明，随着减数分裂进入中期I，极光激酶从组织中心重新定位到减数分裂细胞核，并在着丝粒区域富集。在这里，我们使用双链RNA干扰评估了它们在卵子发生减数分裂中的各自功能。我们发现极光激酶1（Aur1）可能通过调节微管动力学参与减数分裂纺锤体组织和染色体汇聚，而极光激酶2（Aur2）对于染色体凝聚和减数分裂纺锤体组装至关重要。激酶分析表明，Aur1和Aur2具有相当水平的激酶活性。通过酵母双杂交文库筛选，我们为Aur1鉴定了一些新的相互作用蛋白，包括c-Jun氨基末端激酶相互作用蛋白4、黏连蛋白装载因子Scc2和线粒体载体同源物2，这表明Aur1可能具有改变的相互作用网络，并参与微管马达和黏连蛋白复合物的调节。
