Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
J Med Microbiol. 2022 Feb;71(2). doi: 10.1099/jmm.0.001488.
Antimicrobial resistance (AMR) is a One Health issue concerning humans, animals and the environment and a unified One Health approach is required to contain this problematic issue. Dogs and cats are popular pet animals and are known to carry many bacterial pathogens that are of public health importance, including . However, data on AMR in companion animals is limited. Scant AMR data from bacteria originating from companion animals limits an accurate assessment of the impacts of pet-animal-related AMR on public health. This study aimed to phenotypically and genetically investigate AMR in isolated from pet dogs and cats in Thailand. were isolated from pet dogs (=159) and cats (=19) in Thailand between 2016 and 2019. All isolates were serotyped. Phenotypic and genotypic antimicrobial resistance was examined. PCR-based replicon typing, replicon sequence typing and plasmid multilocus sequence typing were conducted to characterize plasmids. Seventy-seven serovars were identified, with serovars Weltevreden (9.6%) and Stockholm (9.0%) the most common. Most of the isolates (34.3%) were multidrug-resistant. The serovar Stockholm was an ESBL-producer and carried the β-lactamase genes and . The plasmid-mediated quinolone resistance (PMQR) gene, was also detected (10.1%). Class 1 integrons carrying the cassette array were most frequent (45.9%). Five plasmid replicon types as IncA/C (0.6%), N (1.1%), IncFIIA (28.7%), IncHI1 (2.2%), and IncI1 (3.4%) were identified. Based on the pMLST typing scheme (=9), plasmids were assigned into five different STs including IncA/C-ST6 (=1), IncH1-ST16 (=4), IncI1-ST3 (=1), IncI1-ST60 (=1) and IncI1-ST136 (=1). The ST 16 of IncHI1 plasmid was a novel plasmid ST. Subtyping F-type plasmids using the RST scheme (=9) revealed four different combinations of replicons including S1:A-:B- (=4), S1:A-:B22 (=2), S3:A-:B- (=1) and S-:A-:B47 (=1). Our findings highlight the role of clinically healthy household dogs and cats as carriers of AMR strains with different R plasmid. The implementation of AMR phenotypes instigation and genotypic monitoring and surveillance programmes in companion animals are imperative as integral components of the One Health framework.
抗微生物药物耐药性（AMR）是一个涉及人类、动物和环境的同一健康问题，需要采取统一的同一健康方法来控制这一问题。狗和猫是受欢迎的宠物动物，已知携带许多对公共卫生很重要的细菌病原体，包括 。然而，关于伴侣动物中的 AMR 数据有限。来自伴侣动物的细菌的 AMR 数据很少，限制了对与宠物相关的 AMR 对公共卫生影响的准确评估。本研究旨在对泰国宠物犬和猫中分离的 进行表型和遗传研究。2016 年至 2019 年间，从泰国的宠物犬（=159）和猫（=19）中分离出 。对所有分离株进行血清型鉴定。检测了表型和基因型抗微生物药物耐药性。进行了基于 PCR 的复制子分型、复制子序列分型和质粒多位点序列分型，以表征质粒。鉴定了 77 种血清型，其中 Weltevreden（9.6%）和 Stockholm（9.0%）血清型最为常见。大多数分离株（34.3%）为多药耐药株。Stockholm 血清型是 ESBL 生产者，携带 β-内酰胺酶基因 和 。还检测到质粒介导的喹诺酮耐药（PMQR）基因 （10.1%）。携带 盒阵列的类 1 整合子最为常见（45.9%）。鉴定出五种质粒复制子类型，包括 IncA/C（0.6%）、N（1.1%）、IncFIIA（28.7%）、IncHI1（2.2%）和 IncI1（3.4%）。根据 pMLST 分型方案（=9），将质粒分为五个不同的 ST，包括 IncA/C-ST6（=1）、IncH1-ST16（=4）、IncI1-ST3（=1）、IncI1-ST60（=1）和 IncI1-ST136（=1）。IncHI1 质粒的 ST16 是一种新的质粒 ST。使用 RST 方案（=9）对 F 型质粒进行亚分型，揭示了四种不同的复制子组合，包括 S1:A-:B-（=4）、S1:A-:B22（=2）、S3:A-:B-（=1）和 S-:A-:B47（=1）。我们的研究结果强调了临床健康的家养犬和猫作为具有不同 R 质粒的 AMR 菌株携带者的作用。在伴侣动物中实施 AMR 表型激发和基因型监测和监测计划是同一健康框架的必要组成部分。
