Department of Oral and Maxillofacial Surgery, Lagos University Teaching Hospital, Lagos, Nigeria.
Department of Oral and Maxillofacial Pathology/Biology, Faculty of Dental Sciences, College of Medicine; University of Lagos, Lagos, Nigeria.
West Afr J Med. 2022 Jan 31;39(1):31-38.
Tissue processing describes the steps involved in infiltrating a well fixed tissue with wax for section cutting on a microtome. Paraffin tissue processing can be performed either manually or by using an automated tissue processor and errors in any of the steps involved would significantly affect histopathology. The aim of this article was to describe to principles of manual tissue processing as performed in the Oral and Maxillofacial Pathology Laboratory of the College of Medicine, University of Lagos, Nigeria (OMFP-CMUL Lab).
We report the principles of manual tissue processing as performed in the OMFP-CMUL lab using the index case of an 11-year-old girl with a histopathologic diagnosis of unicystic ameloblastoma.
Steps of tissue processing included fixation, specimen accessioning, gross examination, decalcification (for tissue specimens containing calcium deposits), dehydration, clearing, infiltration, embedding, sectioning, labeling of tissue blocks, tissue floatation bathing, making the slide and numbering, staining, drying, cover-slipping, and microscopic examination.
Common errors seen in histopathology laboratories were avoided by the quality control practices of the residents, consultants, laboratory scientists and technicians within the department.
组织处理描述了在石蜡切片机上对固定良好的组织进行蜡浸透切片的过程。石蜡组织处理可以手动进行，也可以使用自动化组织处理器进行，如果任何步骤出现错误，都会对组织病理学产生重大影响。本文旨在描述尼日利亚拉各斯大学医学院口腔颌面病理学实验室（OMFP-CMUL 实验室）中进行的手动组织处理原则。
我们报告了在 OMFP-CMUL 实验室中使用一名 11 岁女孩的个案进行手动组织处理的原则，该女孩的组织病理学诊断为单囊型成釉细胞瘤。
组织处理的步骤包括固定、标本登记、大体检查、脱钙（针对含有钙沉积物的组织标本）、脱水、透明、浸透、包埋、切片、组织块标记、组织漂浮浴、制作载玻片和编号、染色、干燥、盖玻片覆盖和显微镜检查。
该部门的住院医师、顾问、实验室科学家和技术人员通过质量控制实践避免了组织病理学实验室中常见的错误。
