Department of Pathology and Laboratory Medicine, Western University and London Health Sciences Centre, London, ON, Canada.
Methods Mol Biol. 2022;2422:47-63. doi: 10.1007/978-1-0716-1948-3_4.
Tissue processing is the technique by which fixed tissues are made suitable for embedding within a supportive medium such as paraffin, and consists of three sequential steps: dehydration, clearing, and infiltration. In most clinical and research settings, tissue processing is accomplished using an automated tissue processor, with or without microwave-assistance. To ensure high-quality results, processing protocols should be tailored to tissue size and composition by modifying variables such as reagents used and the timing of the various steps. Herein, we provide an overview of tissue processing theory and outline a basic tissue processing method for use with a conventional automated fluid transfer/enclosed processor. The principles described will assist readers in optimizing tissue processing for their own projects.
组织处理是将固定组织制成适合嵌入支持介质（如石蜡）的技术，它包括三个连续的步骤：脱水、透明化和渗透。在大多数临床和研究环境中，组织处理是使用自动化组织处理器完成的，无论是否使用微波辅助。为了确保高质量的结果，处理方案应根据组织的大小和组成进行调整，例如改变使用的试剂和各个步骤的时间。本文概述了组织处理理论，并为使用传统的自动液体转移/封闭处理器提供了一种基本的组织处理方法。描述的原理将帮助读者为自己的项目优化组织处理。
