Department of Cardiology, Jinhua People's Hospital, Zhejiang, China.
Nurs Health Sci. 2022 Mar;24(1):312-321. doi: 10.1111/nhs.12927. Epub 2022 Feb 28.
Bone marrow-derived mesenchymal stem cells (BMSCs) and their exosomes are of great significance for the recovery of cardiac function in patients with myocardial infarction (MI). However, the underlying mechanisms of BMSCs applied to MI treatment remain unclear. Fluorescence-activated cell sorting (FACs) are performed to assess the apoptosis, reactive oxygen species levels and glucose uptake capacity of BMSCs. Reverse transcription polymerase chain reaction is conducted to detect the levels of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), insulin-like growth factor (IGF), transforming growth factor-beta 1, connective tissue growth factor, and platelet-derived growth factor. The levels of apoptosis-related proteins were detected by Western blot. The levels of VEGF, bFGF, HGF, and IGF were assessed by enzyme-linked immunosorbent assay. The biochemical kits are applied to detect the levels of malondialdehyde, superoxide dismutase, and adenosine triphosphate/adenosine diphosphate. 2,3,5-triphenyltetrazolium and Masson staining and immunofluorescence are performed to assess myocardial function of rats. Angiopoietin-like protein 4 (ANGPTL4) alleviates apoptosis and oxidative stress of BMSCs induced by serum deprivation and hypoxia; ANGPTL4 activates paracrine and accelerate metabolic energy of BMSCs; and ANGPTL4 treated-BMSCs alleviate myocardial injury of rats with MI. ANGPTL4 treated-BMSCs alleviate myocardial injury in rats with MI, indicating the combination therapy of ANGPTL4 and BMSCs may alleviate myocardial injury in rats with MI.
骨髓间充质干细胞（BMSCs）及其外泌体对心肌梗死后患者心功能的恢复具有重要意义。然而，BMSCs 应用于心肌梗死治疗的潜在机制尚不清楚。通过荧光激活细胞分选（FACs）评估 BMSCs 的凋亡、活性氧水平和葡萄糖摄取能力。通过逆转录聚合酶链反应检测血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）、肝细胞生长因子（HGF）、胰岛素样生长因子（IGF）、转化生长因子-β1、结缔组织生长因子和血小板衍生生长因子的水平。通过 Western blot 检测凋亡相关蛋白的水平。通过酶联免疫吸附测定评估 VEGF、bFGF、HGF 和 IGF 的水平。应用生化试剂盒检测丙二醛、超氧化物歧化酶和三磷酸腺苷/二磷酸腺苷的水平。进行 2,3,5-三苯基四氮唑和 Masson 染色以及免疫荧光染色以评估大鼠的心肌功能。血管生成素样蛋白 4（ANGPTL4）可减轻血清剥夺和缺氧诱导的 BMSCs 凋亡和氧化应激；ANGPTL4 激活旁分泌并加速 BMSCs 的代谢能量；ANGPTL4 处理的 BMSCs 可减轻 MI 大鼠的心肌损伤。ANGPTL4 处理的 BMSCs 可减轻 MI 大鼠的心肌损伤，表明 ANGPTL4 和 BMSCs 的联合治疗可能减轻 MI 大鼠的心肌损伤。
