在失能的大肠杆菌中，通过异源转运系统，由糖的外流驱动的氨基酸人工诱导主动运输。
Artificially induced active transport of amino acid driven by the efflux of a sugar via a heterologous transport system in de-energized Escherichia coli.
作者信息
Bentaboulet M, Robin A, Kepes A
出版信息
Biochem J. 1979 Jan 15;178(1):103-7. doi: 10.1042/bj1780103.
Abstract
Consistent with the model of an H+ cotransport, amino acid uptake can be driven by a proton gradient generated by an efflux of sugar when the normal energy sources are suppressed. Heterologous countertransport is completely inhibited by uncouplers unlike homologous countertransport. Positive coupling was obtained with methyl thiogalactoside/proline, methyl thiogalactoside/phenylalanine, gluconate/proline; however, the poor coupling efficiency suggests a more complex sequence of reactions.
摘要
与H⁺共转运模型一致，当正常能量来源受到抑制时，氨基酸摄取可由糖外流产生的质子梯度驱动。与同源逆向转运不同，异源逆向转运完全被解偶联剂抑制。甲基硫代半乳糖苷/脯氨酸、甲基硫代半乳糖苷/苯丙氨酸、葡萄糖酸盐/脯氨酸获得了正向偶联；然而，较差的偶联效率表明反应序列更为复杂。
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