Department of Physiology, University of Puerto Rico School of Medicine, San Juan, PR 00936, USA.
Alzheimer's Center at Temple, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, USA.
Cells. 2022 Jan 22;11(3):373. doi: 10.3390/cells11030373.
Alzheimer's disease (AD) includes the formation of extracellular deposits comprising aggregated β-amyloid (Aβ) fibers associated with oxidative stress, inflammation, mitochondrial abnormalities, and neuronal loss. There is an associative link between AD and cardiac diseases; however, the mechanisms underlying the potential role of AD, particularly Aβ in cardiac cells, remain unknown. Here, we investigated the role of mitochondria in mediating the effects of Aβ and Aβ in cultured cardiomyocytes and primary coronary endothelial cells. Our results demonstrated that Aβ and Aβ are differently accumulated in cardiomyocytes and coronary endothelial cells. Aβ had more adverse effects than Aβ on cell viability and mitochondrial function in both types of cells. Mitochondrial and cellular ROS were significantly increased, whereas mitochondrial membrane potential and calcium retention capacity decreased in both types of cells in response to Aβ. Mitochondrial dysfunction induced by Aβ was associated with apoptosis of the cells. The effects of Aβ on mitochondria and cell death were more evident in coronary endothelial cells. In addition, Aβ and Aβ significantly increased Ca -induced swelling in mitochondria isolated from the intact rat hearts. In conclusion, this study demonstrates the toxic effects of Aβ on cell survival and mitochondria function in cardiac cells.
阿尔茨海默病（AD）包括细胞外沉积物的形成，这些沉积物包含与氧化应激、炎症、线粒体异常和神经元丧失相关的聚集β-淀粉样蛋白（Aβ）纤维。AD 与心脏疾病之间存在关联；然而，AD 特别是 Aβ 在心脏细胞中潜在作用的机制尚不清楚。在这里，我们研究了线粒体在介导 Aβ 和 Aβ 在培养的心肌细胞和原代冠状动脉内皮细胞中的作用。我们的结果表明，Aβ 和 Aβ 在心肌细胞和冠状动脉内皮细胞中的积累方式不同。与 Aβ 相比，Aβ 对两种细胞的细胞活力和线粒体功能都有更不利的影响。两种细胞的线粒体和细胞 ROS 显著增加，而线粒体膜电位和钙保留能力下降。Aβ 诱导的线粒体功能障碍与细胞凋亡有关。Aβ 对线粒体和细胞死亡的影响在冠状动脉内皮细胞中更为明显。此外，Aβ 和 Aβ 显著增加了完整大鼠心脏线粒体中钙诱导的肿胀。总之，这项研究表明 Aβ 对心脏细胞中细胞存活和线粒体功能具有毒性作用。
